高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (1): 225.doi: 10.7503/cjcu20120351

• 高分子化学 • 上一篇    下一篇

姜黄素抑制金属离子诱导的丝素蛋白构象转变及其作用机理

章月虹, 江腾, 赵学舟, 周平   

  1. 聚合物分子工程国家重点实验室, 复旦大学高分子科学系, 上海 200433
  • 收稿日期:2012-04-13 发布日期:2012-12-31
  • 通讯作者: 周平,女,博士,教授,博士生导师,主要从事生物大分子结构与功能研究.E-mail:pingzhou@fudan.edu.cn E-mail:pingzhou@fudan.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20673022, 21074025)和上海市教育委员会科研创新项目(批准号: 12ZZ009)资助.

Inhibitory Effect of Curcumin on Metal Ions-induced Conformational Transition of Silk Fibroin and Its Mechanism

ZHANG Yue-Hong, JIANG Teng, ZHAO Xue-Zhou, ZHOU Ping   

  1. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China
  • Received:2012-04-13 Published:2012-12-31

PDF (PC)

被引次数

6

摘要：

以丝素蛋白(SF)为神经退行性疾病相关蛋白的模型蛋白, 分析了姜黄素(Curcumin)对Zn(Ⅱ)和Cu(Ⅱ)离子诱导的丝素蛋白构象转变的干预作用及其作用机理, 试图探讨姜黄素在神经退行性疾病中的预防与治疗作用. 结果表明, 姜黄素可通过与Cu(Ⅱ)和Zn(Ⅱ)离子络合, 干预金属离子诱导的丝素蛋白构象转变, 并且所形成的络合物Cu(Ⅱ)-Curcumin可抑制丝素蛋白的构象转变, 但是Zn(Ⅱ)-Curcumin络合物不具有这种能力. 因此, 基于姜黄素对于金属离子诱导的丝素蛋白构象转变具有良好的干预作用, 可以将姜黄素作为神经退行性疾病预防和治疗的潜在候选药物.

关键词: Cu(Ⅱ)离子, Zn(Ⅱ)离子, 姜黄素, 丝素蛋白, 构象转变, 圆二色谱法, 抑制效应

Abstract:

The inhibitory effect of curcumin on the Cu(Ⅱ) and Zn(Ⅱ)-induced conformation transition of silk fibroin(SF) as a model protein of neurodegenerative diseases, was investigated for aiming at investigating the potency of curcumin in the prevention and treatment of neurodegenerative diseases. The results indicate that curcumin can inhibit Cu(Ⅱ) and Zn(Ⅱ)-induced conformational transition of SF through chelating Cu(Ⅱ) and Zn(Ⅱ), meanwhile, Cu(Ⅱ)-curcumin complex also has ability to inhibit the conformation transition of SF, but Zn(Ⅱ)-curcumin complex does not. Therefore, based on those results about the effective interference of curcumin on Cu(Ⅱ) and Zn(Ⅱ)-induced conformation transition of SF, curcumin could be suggested as a potential agent for the prevention and treatment of neurodegenerative diseases.

Key words: Cu(Ⅱ) ion, Zn(Ⅱ) ion, Curcumin, Silk fibroin, Conformation transition, Circular dichroism, Inhibitory effect

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