高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (7): 1086.

• 研究论文 • 上一篇    下一篇

螯合剂对稀土-转铁蛋白配合物的影响(Ⅱ)——稀土-转铁蛋白配合物的阴离子结合性质

杨斌盛, 李英奇   

  1. 山西大学分子科学研究所, 太原 030006
  • 收稿日期:2000-06-14 出版日期:2001-07-24 发布日期:2001-07-24
  • 通讯作者: 杨斌盛(1957年出生),男,硕士,教授,从事生物无机化学研究.
  • 基金资助:

    国家自然科学基金(批准号:20071022);山西省自然科学基金(批准号:991013)

Effect of Chelate Reagents on Terbium-apotransferrin (Ⅱ)——The Properties of Anions Binding to Terbium-apotransferrin

YANG Bin-Sheng, LI Ying-Qi   

  1. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
  • Received:2000-06-14 Online:2001-07-24 Published:2001-07-24

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摘要： 在0.1mol/LHepes,pH 7.4 的条件下,使用紫外差谱和铽(Ⅱ)荧光光谱观察了一些螯合剂对稀土-转铁蛋白配合物REC-apoTf中RE的脱除.荧光滴定表明,螯合剂对TbC-apoTf中铽(Ⅱ)的脱除按缔合机理进行.当螯合剂为非伴阴离子时,脱除过程有TbC-apoTf-L三元配合物中间体生成;当螯合剂为伴阴离子时,脱除过程有L-TbC-apoTf三元配合物中间体生成;L-TbC-apoTf比TbC-apoTf-L更有利于脱除反应的进行;HEDTA,EDTA和NTA可定量地将TbC-apoTf转化为Tb-L或Tb-L2小分子配合物.

关键词: 转铁蛋白, 铽(Ⅱ)离子, 紫外差谱, 荧光光谱

Abstract: The removal of terbium from terbium apotransferrin by the chelate reagents, EDTA, HEDTA, CDTA, CIT, NTA, EDDA, IDAand SUC, were studied in 0.1mol/L N-(2-hydroxyethyl) piperazine-N'-2-ethanesulfonic acid and pH7.4 using UVdifferential spectra and fluorescence spectroscopies. Terbium fluorescence at 549 nm can be used to monitor the change from terbium-apotransferrin to terbium chelate. In the process of terbium removal there exists an intermediate, which is a ternary complex formed from terbium-apotransferrin (Tb-CapoTf) and the chelate reagent (L). When Lis a synergistic anion, the ternary complex is L-Tb-CapoTf while the ternary complex is Tb-CapoTf-Lwhen Lis a nonsynergistic anion. For the removal of terbium L-Tb-CapoTf is more effective than Tb-CapoTf-L. HEDTA, EDTA, NTAcan make Tb-CapoTf change into Tb-Lor Tb-L2 completely.

Key words: Serum transferrin, Terbium ion, UV differential spectroscopy, Fluorescence spectroscopy

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