高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (6): 928.

• 研究简报 • 上一篇    下一篇

采用手持式光度计的△I测量模式快速测定尿样中白蛋白

陈焕文1, 金钦汉1, 杨立泉2, 罗贵民2   

  1. 1. 吉林大学化学系, 长春分析仪器研究和技术开发中心, 长春 130023;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春 130023
  • 收稿日期:2000-05-20 出版日期:2001-06-24 发布日期:2001-06-24
  • 通讯作者: 金钦汉(1937年出生),男,教授,博士生导师,主要从事光谱分析和分析仪器研究.
  • 基金资助:

    吉林省科学技术厅(批准号:吉科合字第20000105号)资助

Rapid Determination of Albumin of Urine in ΔI Measurement Mode with Portable Spectrophotometer of High Sensitivity

CHEN Huan-Wen1, JIN Qin-Han1, Yang-Li-Quan2, Luo-Gui-Min2   

  1. 1. Department of Chemistry, Jilin University, Changchun R & D Centerf or Analytical Instruments, Changchun 130023, China;
    2. Education Ministry Key Laboratory of Molecular Enzy mologyand Engineering, Jilin University, Changchun 130023, China
  • Received:2000-05-20 Online:2001-06-24 Published:2001-06-24

关键词: 手持式光度计, &Delta, I测量模式, 白蛋白, 尿样, 快速测定

Abstract: Aportable spectrophotometer developed by Changchun R & D Center for Analytical Instruments, which combines ΔImeasurement mode and a measurement mode together with a high sensitivity, good accuracy and low cost, is employed to replace the traditional spectrophotometer and the sensitivity is enhanced. Therefore, a new rapid method with low cost and simplicity in operation for protein determination is developed. For this method, the linear range is shown to be 0—0.74 μg/mL, the detection limit is 0.01 μg/mL, and the analysis can be completed within 5 min. The albumin in urine is determined and all the results are confirmed by the standard method. Because of the rapid analysis speed, less expenditure, more convenience and simplicity in operation of this method, it is possible to use this method to diagnose glycosuric nephropathy at an early stage, even do it at home.

Key words: Portable spectrophotometer, ΔI measurement mode, Albumin, Urine, Rapid determination

中图分类号: 

TrendMD: