高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (12): 2100.

• 研究论文 • 上一篇    下一篇

用低交换容量聚苯乙烯型强酸性阳离子交换树脂柱色谱分离中性氨基酸

刘菊湘1, 刘国栋1, 阎虎生1, 程晓辉1, 何炳林1, 姜根华2, 杨国英2   

  1. 1. 南开大学高分子化学研究所, 吸附与分离功能高分子材料国家重点实验室, 天津 300071;
    2. 天津药业有限公司, 天津 300161
  • 收稿日期:2000-10-10 出版日期:2001-12-24 发布日期:2001-12-24
  • 通讯作者: 阎虎生(1959年出生),男,博士,教授,博士生导师,从事功能高分子和多肽合成研究.E-mail:h.s.yan@eyou.com E-mail:h.s.yan@eyou.com
  • 基金资助:

    天津市重点科学基金项目(批准号:983801411)资助

Separation of Neutral Amino Acids on Columns Packed with Polystyrene-Based Strongly Acidic Cation Exchange Resins with Low Ion Exchange Capacity

LIU JuXiang1, LIU GuoDong1, YAN HuSheng1, CHENG XiaoHui1, HE BingLin1, JIANG GenHua2, YANG GuoYing2   

  1. 1. State Key Laboratoryof Functional Polymer Materialsf or Adsorptionand Separation, Instituteof Polymer Chemistry, Nankai University, Tianjin 300071, China;
    2. Tianjin Pharmaceutical Company, Tianjin 300161, China
  • Received:2000-10-10 Online:2001-12-24 Published:2001-12-24

摘要: 通过磺化苯乙烯-二乙烯苯共聚物或商品化的聚苯乙烯型强酸性阳离子交换树脂(001×7)的逆磺化反应,得到一系列不同交换容量的聚苯乙烯型强酸性阳离子交换树脂.研究了丙氨酸和缬氨酸及缬氨酸和亮氨酸在这些树脂柱上的色谱分离.结果表明,用两种方法得到的树脂对丙氨酸和缬氨酸的色谱分离性能基本相同;同时中性氨基酸与聚苯乙烯型强酸性阳离子交换树脂之间的作用包括离子作用和疏水作用,且二者之间存在协同作用.树脂的交换容量较低时对中性氨基酸有更好的分离性能.

关键词: 氨基酸, 离子交换树脂, 疏水作用, 色谱

Abstract: Aseries of polystyrenebased strongly acidic cation exchange resins with different ion exchange capacities were synthesized via the reversedsulfonation of a commercial polystyrenebased strongly acidic cation exchange resin(001×7) or sulfonation of styrenedivinylbenzene copolymer. The chromatographic separation of alanine and valine or valine and leucine on columns packed with these resins was carried out. The results showed that the chromatographic properties of the two kinds of the abovementioned resins on the separation of alanine and valine were almost the same. The retention volume of alanine was less than that of valine on the same column with various ion exchange capacities, and the retention volume of valine was less than that of leucine on the column with the ion exchange capacity of 1.67 mmol/mL. The retention volume of alanine decreased when the ion exchange capacities decreased from 2.02 to 1.00 mmol/mL. The retention volume of valine slightly decreased when the ion exchange capacities decreased from 2.02 to 1.67 mmol/mLand remained almost unchanged when the ion exchange capacities decreased from 1.67 to 1.00 mmol/mL. These results indicated that the interaction between neutral amino acids and the polystyrenebased strongly acidic cation exchange resin includes ionic and hydrophobic interactions. And there may be synergy between the two interactions. The better separation efficiency of neutral amino acid may be obtained by using the resin with a lower ion exchange capacity.

Key words: Amino acid, Ion exchange resin, Hydrophobic interaction, Chromatography

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