关键词: 极性, 壳聚糖膜, 芘, 荧光探针

Abstract: A medium polarity sensitive membrane was prepared by embedding pyrene(Py), a polarity fluorescence probe, into a glutaraldehyde(Glu) crosslinked chitosan(CS) membrane. The composition and thermostability of the membrane were characterized by FTIRand TGAanalysis. It is shown that the composition of the membrane is close to that of CS. The introduction of micro amount of Py and crosslinker has little effect upon the composition of the membrane. However, the Glu crosslinked CSmembrane is more stable toward heating than the uncrosslinked CSmembrane. In addition, the crosslinked CSmembrane is also stable in acidic and basic media. The CS-Glu membrane containing Py was successfully used to monitor the polarities of various solvents including water, ethanol, and ethanol water mixtures. Based upon the measurement of polarity, the prepared membrane was also used to monitor the micellar formation in SDSsolution. Compared with the traditional solution fluorescence probing method, this new method is more simple and convenient, and can be recycled.

Key words: Polarity, Chitosan membrane, Pyrene, Fluorescence probe