高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (7): 1018.

• 论文 • 上一篇    下一篇

改进溶胶-凝胶法固定酶结构剖析及在苯酚光化学传感器中的应用

李军, 王柯敏, 肖丹, 羊小海   

  1. 湖南大学化学化工学院, 长沙 410082
  • 收稿日期:1999-08-05 出版日期:2000-07-24 发布日期:2000-07-24
  • 通讯作者: 王柯敏(1957年出生),男,博士,博士生导师,从事光化学传感器研究.
  • 基金资助:

    国家自然科学基金(批准号:29675005);国家杰出青年基金(批准号:29825110)资助

The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor

LI Jun, WANG Ke-Min, XIAO Dan, YANG Xiao-Hai   

  1. College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China
  • Received:1999-08-05 Online:2000-07-24 Published:2000-07-24

摘要: 通过光学显微镜比较了用密闭老化法和浸涂法制备的溶胶凝胶膜的表面状态,从实验上证实了用密闭老化法制备的溶胶凝胶膜不会破裂;并用透射电子显微镜观察了包埋辣根过氧化物酶的溶胶凝胶膜的内部结构.结果表明,酶固定于溶胶凝胶中后,与在溶液中一样,呈均匀分布且不易流失.此敏感膜可用于制备基于化学发光强度减弱的苯酚光化学传感器;用竞争反应的原理讨论了响应机理.

关键词: 溶胶-凝胶, 辣根过氧化物酶, 微观结构, 苯酚, 光化学传感器

Abstract: The external states of horseradish peroxidase(HRP) encapsulated membrane prepared by enclosed aging method and dip coating method are compared by common optical microscopy to clarify that the sol gel membrane prepared by enclosed aging method is no cracking. The transmission electron microscopy(TEM) is used to observe the character of the sol gel derived HRPmembrane. The TEMphotos show that enzyme can uniformly distribute in the gel as if in the solution and do not leach from the gel. This membrane can be used to fabricate a phenol optical sensor based on the decrease of chemiluminescence intensity. The response mechanism is discussed by compete reaction.

Key words: Sol gel, Horseradish peroxidase, Microstructure, Phenol, Optical sensor

中图分类号: 

TrendMD: