高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (6): 961.

• 论文 • 上一篇    下一篇

灵芝菌丝体多糖的化学组成和溶液性质

陈敬华1, 张俐娜1, 余登寿2, 朱荣萍3   

  1. 1. 武汉大学化学系, 武汉大学生命科学院, 武汉 430072;
    2. 国家标准物质研究中心, 北京 100013;
    3. 国家标准物质研究中心, 北京 100013
  • 收稿日期:1999-05-11 出版日期:2000-06-24 发布日期:2000-06-24
  • 通讯作者: 张俐娜(1940年出生),女,教授,博士生导师,从事天然多糖溶液和纤维素改性膜结构与性能的研究.E-mail:Lnzh ang@p ublic.wh.hb.cn E-mail:Lnzh ang@p ublic.wh.hb.cn
  • 基金资助:

    国家自然科学基金(批准号:29374170)

Chemical Components and Solution Properties of a Glycan-Protein Complex from Ganoderma Lucidum Mycelium

CHEN Jing-Hua1, ZHANG Li-Na1, YU Deng-Shou2, ZHU Rong-Ping3   

  1. 1. Department of Chemistry;
    2. College of Life Science, Wuhan University, Wuhan 430072;
    3. Research Center of Certified Materials of China, Beijing 100013, China
  • Received:1999-05-11 Online:2000-06-24 Published:2000-06-24

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被引次数

32

摘要： 用磷酸盐缓冲液在80℃时从灵芝菌丝体中提取出多糖-蛋白质缀合物LM-A.成分分析表明,GLM-A主要由葡萄糖组成,蛋白质含量为13%.GLM-A在1∶1水稀释的饱和镉乙二胺溶液中30℃时的特性粘数[η]和均方根旋转半径〈S2〉1/2的分子量依赖关系分别为[η]=5.1×10-2Mw0.06(cm3·g-1)和〈S2〉1/2=3.9×10-2Mw0.50(nm).按照Yamakawa-Fujii-Yoshizaki蠕虫状圆筒模型的粘度理论和Bohdanecky表达式,求得GLM-A的分子参数为:单位围长摩尔质量ML=(530±10)nm-1,持久长度q=(2.8±0.2)nm.链直径d=0.75nm.实验结果表明,GLM-A在该溶液中为无规线团构象.

关键词: 灵芝菌丝体, 化学组成, 分子量, 链刚性, 溶液性质

Abstract: A glycan-protein complex (GLM-A) was isolated from the Ganoderma lucidum with phosphate buffer solution at 80 ℃. The carbohydrate in GLM-Ais almost constituted by glucose, and the content of protein is about 13%. The molecular weight Mw dependences of intrinsic viscosity [η] and root mean square radius of gyration 〈 S2〉1/2 of GLM Ain the 1∶1 water diluted cadoxen at 30 ℃ are [ η ]=5.1×10-2 Mw0.60 (cm3·g-1 ) and 〈 S2〉1/2=3.9×10-2Mw0.50(nm), respectively. According to Bohdanecky's method based on Yamakawa Fujii Yoshizakis theory for worm like chain cylinder, molar mass per unit contour length M L, persistence length q , and chain diameter d were estimated to be (530±10) nm-1 , (2.8±0.2) nm, and 0.75 nm, respectively, in the 1∶1 water-diluted cadoxen. The results indicated that the glycan protein complex exists as a random coil in the aqueous solution.

Key words: Ganoderma lucidum mycelia, Chemical component, Molecular weight, Chain stiffness, Solution property

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