高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (6): 881.

• 研究简报 • 上一篇    下一篇

PAP-H2O2-HRP伏安酶联免疫分析新体系测定人血清总甲状腺素

张书圣1, 焦奎2, 陈洪渊1   

  1. 1. 南京大学化学系, 南京 210093;
    2. 青岛化工学院应用化学系, 青岛 266042
  • 收稿日期:1998-10-28 出版日期:1999-06-24 发布日期:1999-06-24
  • 通讯作者: 焦奎
  • 作者简介:张书圣,男,32岁,副教授,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29775012);长春应用化学研究所电化学开放实验室基金

PAPH2O2HRP Voltammetric Enzymelinked Immunoassay for Trace Thyroxine in Human Serum

ZHANG Shu-Sheng1, JIAO Kui2, CHEN Hong-Yuan1   

  1. 1. Department of Chemistry, Nanjing University, Nanjing, 210093;
    2. Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Qingdao, 266042
  • Received:1998-10-28 Online:1999-06-24 Published:1999-06-24

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摘要： 目前临床检测中测定总甲状腺素(T4)的常用方法有间接血凝试验、琼脂双扩散及ELISA等方法[1].其中ELISA法是目前较为流行的检测方法,但灵敏度不高.伏安酶联免疫分析法具有广阔的应用前景[2,3].

关键词: 对氨基酚, 辣根过氧化物酶, 免疫分析, 伏安法, 总甲状腺素(T4)

Abstract: A voltammetric enzymelinked immunoassay based on new system of p-aminophenol(PAP)H2O2-horseradish peroxidase(HRP) has firstly been developed and used for the detection of HRP and thyroxine(T4). HRP or labelled HRP catalyzes the oxidation reaction of PAP with H2O2, the product of which produces a sensitive voltammetric peak at potential of -0.45 V(vs. SCE) in BrittonRobinson(B-R) buffer solution. By using this voltammetric peak, the detection limit to thyroxine(T4)is 10 ng/mLand the linear range 1.0-320 ng/mL. The detection limit to T4with this method is lower than that with the enzymelinked immunosorbent spectrophotometric assay(ELISA). Some human serum samples have been analyzed and satisfactory results have been obtained.

Key words: p-Aminophenol, Horseradish peroxidase(HRP), Immunoassay, Voltammetry, Thyroxine(T4)

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