高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (2): 167.

• 论文 •    下一篇

氧钒离子作用下人血红蛋白构象变化的动态过程及2,3-二磷酸甘油酸的影响

薛德平1, 林华宽2, 王夔3   

  1. 1. 南京大学化学化工学院, 南京 210008;
    2. 南开大学化学化工学院, 天津 300071;
    3. 北京医科大学无机药物及生物无机化学系, 北京 100083
  • 收稿日期:1998-01-05 出版日期:1999-02-24 发布日期:1999-02-24
  • 通讯作者: 王夔
  • 基金资助:

    国家自然科学基金(批准号:29471007)

The Process of Conformational Change of Human Hemoglobin Induced by VanadyLIon Binding and the Effect of 2,3-Diphosphoglycerate

XUE De-Ping1, LIN Hua-Kuan2, WANG Kui3   

  1. 1. Department of Chemistry, Nanjing University, Nanjing, 210093;
    2. Department of Chemistry, Nankai University, Tianjin, 300071;
    3. Department of Bioinorganic & Inorganic Medical Chemistry, Beijing Medicine University, Beijing, 100083
  • Received:1998-01-05 Online:1999-02-24 Published:1999-02-24

PDF (PC)

被引次数

8

摘要： 用停流分光光度计检测在有或无DPG结合时Hb与氧钒离子作用过程中自身荧光的变化,发现无DPG的Hb自身荧光只是一个单调的荧光猝灭过程.DPG结合于Hb后的荧光变化则先为荧光猝灭,转而缓慢增加.用氧钒离子结合引起的构象变化及DPG和DPG的水解产物3PGA所起的稳定Hb四级结构作用解释了上述过程.

关键词: 氧钒离子, 人血红蛋白, 2, 3-二磷酸甘油酸, 构象变化

Abstract: The process of the conformational change of human hemoglobin as the result of its interaction with vanadyLIons with and without DPG was determined by means of stopped flow measurement of the intrinsic fluorescence of Hb. The vanadyl binding causes monotonous quenching of the intrinsic fluorescence of DPG depleted Hb. It is noteworthy that the binding of DPG to Hb makes the process to be biphasic one. The first step is also featured by the quenching of intrinsic fluorescence. In the second step of DPG Hb system, the intrinsic fluorescence stops to decrease and turns to increase very slightly. The process was explained on the basis of vanadylbinding induced conformation change and the stabilizing effect of DPG and its hydrolytic product 3PGA to the tetrameric structure of Hb.

Key words: Vanadyl, Human hemoglobin, 2, 3-Diphosphoglycerate, Conformational change

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