高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (10): 1533.

• 论文 • 上一篇    下一篇

径向电场调制毛细管电泳法用于蛋白质分离

朱英, 陈义   

  1. 中国科学院化学研究所, 北京 100080
  • 收稿日期:1999-01-26 出版日期:1999-10-24 发布日期:1999-10-24
  • 通讯作者: 陈义
  • 作者简介:朱英,女,28岁,博士,现在中国预防医学科学院环境监测研 究所工作.
  • 基金资助:

    国家自然科学基金(批准号:29575215、29635020)资助课题

Capillary Electrophoresis of Proteins Under the Control of Radial Electric Field

ZHU Ying, CHEN Yi   

  1. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
  • Received:1999-01-26 Online:1999-10-24 Published:1999-10-24

PDF (PC)

被引次数

17

摘要： 利用自制的双向电场控制毛细管电泳新系统,考察了蛋白质的分离情况.结果发现,在低pH值下,通过施加径向电场,不仅可改变电渗流的大小和方向,而且能抑制蛋白质的吸附,进而实现对蛋白质分离效率和分离速度的调控.研究结果表明,可通过物理化学方法实现毛细管电泳的动态或随机调控,这对许多生物样品分离有实际意义.

关键词: 毛细管电泳, 蛋白质分离, 径向电场控制

Abstract: Separation of basic proteins was performed using a homemade electric field modu-lated capillary electrophoretic system, which can offer both radial and axial electric fields with only one high voltage power supply. The most interesting phenomenon observed is that the adsorption of protein molecules onto the tubing wall can be regulated by the application of a radial voltage(potential at the outer wall minus that at the inner wall): band broadening decreases with the increase of the radial voltage. This implies that it may be possible to dy-namically control the adsorption of protein molecules on silica surface. In addition, the radial voltage is also able to adjust the resolution of the solutes due to the change of electroosmosis.

Key words: Capillary electrophoresis, Protein separation, Radial electric field control

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