高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (9): 1513.

• 论文 • 上一篇    下一篇

香菇多糖的成分及其分子量研究

张俐娜1, 张平义1, 李翔1, 潘苏1, 朱荣平2   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 国家标准物质研究中心, 北京, 100013
  • 收稿日期:1997-07-15 出版日期:1998-09-24 发布日期:1998-09-24
  • 通讯作者: 张俐娜,女,57岁,教授.
  • 作者简介:张俐娜,女,57岁,教授.
  • 基金资助:

    国家自然科学基金(批准号:29374170)资助课题.

Studies on Components and Molecular Weights of Polysaccharides from Lentinus Edodes

ZHANG Li-Na1, ZHANG Ping-Yi1, LI Xiang1, PAN Su1, ZHU Rong-Ping2   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. National Research Center for Certified Reference Materials, Beijing, 100013
  • Received:1997-07-15 Online:1998-09-24 Published:1998-09-24

摘要: 从香菇子实体中用0.9%NaCl水溶液、85%乙醇、热水、1%草酸铵、5%NaOH/0.05%NaBH4和2%NaOH/2%尿素分别提取出四种杂多糖(L-FⅠ-L-FⅣn)和两种葡聚糖(L-FⅤ和L-FⅥn).红外光谱和高效液相色谱分析结果表明杂多糖主要由葡萄糖、葡萄糖醛酸、木糖、甘露糖、半乳糖和鼠李糖组成,而且按照分离过程的进行多糖中葡萄糖含量递增.由光散射和膜渗透压法研究了多糖的重均分子量Mw、均方根旋转半径<S2>1/2、第二维利系数A2及数均分子量Mn.L-FⅡ、L-FⅢ、L-FⅤ及L-FⅥn的Mw值依次为19.7×104、192.3×104、136.4×104和136.7×104,它们的多分散系数-Mw/-Mn在3~5范围.

关键词: 香菇多糖, 分子量, 高效液相色谱, 光散射, 膜渗透压法

Abstract: Four heteropolysaccharides(L-FⅠ_L-FⅣn ) and two glucans(L-FⅤ and L-FⅥn ) were isolated from the fruit bodies of lentinus edodes by using aqueous 09% NaCl, 85% ethanol, hot water(120 ℃, high pressure), aqueous 1% ammonium oxalate, aqueous 5% NaOH/005% NaBH4 and 2% NaOH/2% urea, respectively. The results analyzed with IRand HPLCshowed that the heteropolysaccharides consist of Dglucose, glucuronic acid, xylose, mannose and galactose and rhamnose, and the D-glucose content in the polysaccharides increases with the progress of isolation process. The weight average molecular weights Mw, root-mean-square radii of gyration 〈S21/2, second Virial coefficients A2 and number average molecular weights Mn of the polysaccharides were studied by using light scattering and membrane osmometry. The Mw values of the samples of L-FⅡ, L-FⅢ, L-FⅤ and L-FⅥ n are 19.7×104, 19.23×104, 13.64×104 and 13.67×104, respectively. The polydispersity indexes of the polysaccharides Mw/ Mn lie in the range of 3~5.

Key words: Lentinan edodes, Molecular weight, HPLC, Light scattering, Membrane osmometry

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