高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (1): 53.

• 研究简报 • 上一篇    下一篇

样品带示踪法测定毛细管电泳过程中的电渗流

傅崇岗, 王安宝, 叶建农, 方禹之   

  1. 华东师范大学化学系, 上海, 200062
  • 收稿日期:1997-01-22 出版日期:1998-01-24 发布日期:1998-01-24
  • 通讯作者: 方禹之
  • 作者简介:傅崇岗,男,32岁,博士研究生.
  • 基金资助:

    中国科学院长春应用化学研究所电分析化学开放实验室基金资助课题.

A New Method for Determination of Electroosmotic Mobility Using Sample Zone as a Marker

FU Chong-Gang, WANG An-Bac, YE Jian-Nong, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1997-01-22 Online:1998-01-24 Published:1998-01-24

关键词: 电渗流淌度, 毛细管电泳, 样品带示踪

Abstract: A new method for the determination of the electroosmotic mobility was presented in this paper. The "sample zone" itself was used as a marker of the electroosmotic flow. The elution time of the sample zone from the capillary was derived from the position of the electrophoretic current step. Based on it the mobility of electroosmotic flow can be calculated.The results was well in agreement with those of the neutral marker method. Furthermore,the present method was easy to operate and need no additive equipments.

Key words: Electroosmotic flow, Capillary electrophoresis, Sample zone marking

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