关键词: 毛细管电泳, 电化学检测, 手性拆分, 氯霉素前体

Abstract: Chiral separation of the enantiomers of threo-2-amino-1-(4-nitrophenyl)-1, 3-propanediol, namely the prodrug of chloramphenicol (PCP), was carried out by capil1aryzone electrophoresis with electrochemical detection employing β-cyclodextrin (β-CD) as achiral additive in strong alkaline solutions.The analytes were detected by electrochemistryusing a copper disk electrode at +675 mVversus Ag/AgCl reference electrode.Both the freeenantiomers and the enantiomer-cyclodextrin inclusion complexes could be detected usingthis approach, although the complexed forms gave lower oxidation currents than the uncomplexed ones.Factors affecting the chiral CEseparation of the analytes, such as concentrationof running buffer and β-CD, applied voltage, were extensively investigated.Under the optimum conditions, baseline separation of the enantiomers could be accomplished in less than l8min.In addition, an attempt was made to elucidate the pIausible mechanism 0f the chiralrecognition.

Key words: Capillary electrophoresis, Electrochemical detection, Chiral separation, Amine derivatives