高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (12): 1847.

• 论文 • 上一篇    下一篇

复合高分子膜修饰电极直接测定大脑内源性神经递质

彭图治1, 杨丽菊1, 李惠萍1, 王国顺1, 洪佳琼2   

  1. 1. 杭州大学化学系, 杭州, 310028;
    2. 浙江医院
  • 收稿日期:1994-12-08 修回日期:1995-03-30 出版日期:1995-12-24 发布日期:1995-12-24
  • 通讯作者: 彭图治,男,48岁,教授.
  • 作者简介:彭图治,男,48岁,教授.
  • 基金资助:

    国家自然科学基金;浙江省自然科学基金

Determination of the Neurotransmitter in the Brain Using Composite Polymer Microelectrodes

PENG Tu-Zhi1, YANG Li-Ju1, LI Hui-Ping1, WANG Guo-Shun1, HONG Jia-Qiong2   

  1. 1. Department of Chemistry, Hangzhou University, Hangzhou, 310028;
    2. Zhejiang Hospital
  • Received:1994-12-08 Revised:1995-03-30 Online:1995-12-24 Published:1995-12-24

摘要: 采用阳离子交换剂Nafion和醋酸纤维素复合修饰的碳纤维电极具有电荷选择性和尺寸选择性。对复杂样品具有独特的选择性和抗干扰性。在动物活体伏安分析中,该复合修饰电极成功地抑制了神经递质代谢产物、抗坏血酸和尿酸等电化学活性物质在电极表面的反应,直接检测了脑内神经递质多巴胺的氧化电流。该电极采用静电浸渍法多次涂布,使电极的复合膜牢固,并具有很好的分析性能。同时,对复合修饰电极的作用机理进行了探讨。

关键词: 微电极, 化学修饰电极, 伏安法, 多巴胺, 神经递质

Abstract: A composite polymer carbon fiber electrode modified with Nafion and cellulose acetate is described.The incorporation of ion exchanging polymer to the cellulose acetate domain allows the binding of counterionic reactants, while maintaining the size exclusion discriminative properties of cellulose acetate.In vivo voltammetry,the modified microelectrode inhabits the oxidation of some neurotransmitter metabolites and electroactive compounds,such as DOPAC, 5-HIAA, HVA, AA and UA;and successfully detects the oxidative current of neurotransmitter DA.

Key words: Microelectrode, Chemically modified electrode, Voltammetry, Dopamine, Neurotransmitter

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