高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (4): 600.

• 研究简报 • 上一篇    下一篇

用共振拉曼光谱研究电子激发态铜卟啉与DNA的相互作用

赵晓杰1, 江山1, 陆冬生1, 陆琳1, 毛慈波1, 安承武1, 范永昌1, 李再光1, 周翔2, 黄素秋2   

  1. 1. 华中理工大学激光技术国家重点实验室, 武汉, 430074;
    2. 武汉大学化学系
  • 收稿日期:1993-06-15 修回日期:1993-09-14 出版日期:1994-04-24 发布日期:1994-04-24
  • 通讯作者: 陆冬生
  • 作者简介:赵晓杰,男,28岁,博士研究生.
  • 基金资助:

    国家教育委员会重点科研基金

Studies on Interaction of Electronically Excited Water-Soluble Copper-Porphyrins with DNA by Resonance Raman Spectroscopy

ZHAO Xiao-Jie1, JIANG Shan1, LU Dong-Sheng1, LU Lin1, MAO Ci-Bo1, AN Cheng-Wu1, FAN Yong-Chang1, LI Zai-Guang1   

  1. National Laboratory of Laser Technology, Huazhong University of Sicence and Technology, Wuhan, 430074
  • Received:1993-06-15 Revised:1993-09-14 Online:1994-04-24 Published:1994-04-24

关键词: 水溶性卟啉, 受激复合物, DNA, 共振拉曼光谱

Abstract: The resonance Raman spectra of water-soluble porphyrin Cu(NACN)[Cu-tetrakis(4-N-acetonitrilepyridyl)porphyrin], Cu(NEAE) and Ni(NEAE)[Cu or Ni-tetrakis (4-N-ethylacetatepyridyl) porphyrin] and their complexes with calf thymus DNAat different laser pulse powers were measured with 445 nm pulse laser excitation; The analysis results indicate that both of the copper-porphyrins formed electronic exciplex with DNAbut the Ni(NEAE)did not form, In this experiment, besides copper-porphyrin Raman bands Ⅵ (near 1370 cm-1)and Ⅷ(near 1570 cm-1).band Ⅶ(near 1470 cm-1) also show extra band which symbolizes the formation of exciplex.The triplet of copper-porphyrin with a long life time is quenched by DNAand the exciplex may be formed by charge transfer from triplet of Cu-porphyrin to DNA.

Key words: Water-soluble porphyrins, Exciplex, DNA, Resonance Raman spectroscopy

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