高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (9): 1229.

• 研究简报 • 上一篇    下一篇

毛细管区带电泳中的pH效应

林炳承1, 施伟1, 胡六江2, 杨国墚2, 黄建福3, 张乃谦3   

  1. 1. 中国科学院大连化学物理研究所;
    2. 杭州大学化学系;
    3. 大连轻工学院食品工程系
  • 收稿日期:1992-09-30 修回日期:1993-05-31 出版日期:1993-09-24 发布日期:1993-09-24
  • 通讯作者: 林炳承
  • 作者简介:第一作者:男, 49岁, 博士, 研究员.
  • 基金资助:

    国家自然科学基金

The Effect of the Buffer pH in Capillary Zone Electrophoresis

LIN Bing-Cheng1, SHI Wei1, HU Liu-Jiang2, YANG Guo-Liang2, HUANG Jian-Fu3, ZHANG Nai-Qian3   

  1. 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116012;
    2. Dept.of Chem., Hangzhou Univ., Hangzhou;
    3. Dalian Inst.of Light Industry, Dalian
  • Received:1992-09-30 Revised:1993-05-31 Online:1993-09-24 Published:1993-09-24

关键词: 毛细管区带电泳, pH影响, 肽, 蛋白质, 吸附

Abstract: The effect of buffer pHon the migration time of both peptides and proteins was studied in the range from pH 2.5 to 111 the dependence of the elute order and resolution of the components on the pHis very sensitive and should be optimized.Special attention was also paied to it's effect on the absorption of the wall on the proteins at the basic condition.It is one of the ways to inhibit the absorption, but the pHvalues should be chosen very carefully.

Key words: Capillary zone electrophoresis, Effect of pH, Peptide, Protein, Absorption

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