高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (7): 940.

• 论文 • 上一篇    下一篇

漆树漆酶催化2,6-二甲基酚的氧化反应

季立才, 胡培植, 徐文中   

  1. 武汉大学化学系, 武汉, 430072
  • 收稿日期:1991-07-15 出版日期:1992-07-24 发布日期:1992-07-24
  • 通讯作者: 季立才

Rhus Vernicifera Laccase Catalyzes Oxidation of 2,6-Dimethylphenol

JI Li-cai, HU Pei-zhi, XU Wen-zhong   

  1. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1991-07-15 Online:1992-07-24 Published:1992-07-24

摘要: 在0.1mol/L磷酸盐缓冲液中,漆树漆酶催化2,6-二甲基酚(DMP)氧化生成3,3'5.5'-四甲基-4,4'-二苯醌(TMDQ).pH8.0,温度40~50℃时酶促氧化DMP较快.DMP浓度在1.66×10-4~9.92×10-4mol/L时为一级反应,速率常数为3.57×10-5s-1,共存的二茂铁(Fc—H)能大大促进酶促氧化DMP的速度.提出了该反应可能的反应机理。

关键词: 漆树漆酶, 2, 6-二甲基酚, 二茂铁, 酶促氧化, 3, 3'5, 5'-四甲基-4, 4'-二苯醌

Abstract: Rhus vernicifera laccase catalyzes the oxidation of 2, 6-dimethylphenol(DMP) to 3, 3', 5,5' -tetramethyl-4,4' -diphenoquinone (TMDQ) in 0.1 rnol/Lphosphate buffer solution.The enzymatic oxidation of DMPis much faster at pH 8.0 and temperature 40~50℃.The reaction is a first-order and the rate constant (kobs) is 3.57× 10-5 s-1 when DMP concentration is in the range of 1.66 × 10-4~9.92 × 10-4 mol/L.The coexistent ferrocene (Fc-H) can more greatly promote the enzymatic oxidation of DMP.The possible mechanism of laccase-catalyzed oxidation of DMPand coexistent Fc-H was inferred.

Key words: Rhus vernicifera laccase, 2,6-Dimethylphenol, Ferrocene, Enzymatic promation oxidation, 3,3'5,5'-Tetramethyl-4,4'-diphenoquinone

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