高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (5): 583.

• 论文 • 上一篇    下一篇

大白鼠脑组织电极测定鸟嘌呤

邓家祺, 孔继烈, 何红   

  1. 复旦大学化学系, 上海, 200433
  • 收稿日期:1991-04-29 出版日期:1992-05-24 发布日期:1992-05-24
  • 通讯作者: 邓家祺
  • 基金资助:

    国家自然科学基金;长春应用化学研究所电分析化学开放实验室资助

A Sensor for Guanine Using Brain Tissue of Rat

DENG Jia-qi, KONG Ji-lie, HE Hong   

  1. Department of Chemistry, Fudan University, Shanghai, 200433
  • Received:1991-04-29 Online:1992-05-24 Published:1992-05-24

摘要: 以大白鼠脑组织作催化鸟嘌呤脱氨反应的酶源,采用氨气敏电极为基础电极制成测定鸟嘌呤的大白鼠脑组织电极,对其响应特性、介质条件、固定化方法等因素进行了研究,测定鸟嘌呤的E~log[鸟嘌呤]曲线斜率为46.5mV,线性范围2.0×10-5~6.3×10-4mol/L,寿命可达28d。

关键词: 大白鼠脑, 鸟嘌呤, 组织电极, 生物传感器

Abstract: An electrode for the determination of guanine was prepared, which employed brain tissue of rat as the biocatalyst and an ammonia gas sensing electrode as the base electrone.Its response characteristics, buffer conditions and strategies of immobilization were studied.The slope of guanine response curve E-lg[Guanine] is 46.5 mV/decade, linear range is 2.0×10-5~6.3×10-4 mol/L, lifetime is over 28 days.

Key words: Brain tissue of rat, Guanine, Tissue based electrode, Biosensor

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