高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (6): 754.

• 论文 • 上一篇    下一篇

1,4-二巯基苏糖醇的极谱研究

曾百肇, 查健, 周性尧   

  1. 武汉大学化学系, 武汉, 430072
  • 收稿日期:1990-01-24 出版日期:1991-06-24 发布日期:1991-06-24
  • 通讯作者: 周性尧
  • 基金资助:

    国家自然科学基金

Studies on Polarographic Behavior of 1,4-Dithiothreitol

Zeng Bai-zhao, Zha Jian, Zhou Xing-yao   

  1. Department of Chemistry, Wuhau University, Wuhan, 430072
  • Received:1990-01-24 Online:1991-06-24 Published:1991-06-24

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被引次数

3

摘要： 1,4-二巯基苏糖醇(简称DTT)是一种重要的生化物质,广泛地应用于蛋白质的分离、测定和生理作用的研究等方面。尽管DTT在生物医学上有重要的作用,但它的分析测定方法报道的却不多,其中电分析方法更为少见。本文研究了它的极谱性质,发现以氯乙酸为介质,在单扫描示波极谱图上DTT产生灵敏的吸附波,此波可用于痕量DTT的测定。

关键词: 1, 4-二巯基苏糖醇, 极谱吸附波, 巯基化合物极谱性质

Abstract: In ClCH2COOH medium, 1,4-dithiothreitol (DTT) can shows a sensitive polarographic wave at -0. 31 V (vs. SCE). In this paper, conditions are found for the determination of 1,4-dithiothreitol with single sweeping polarography. Under the best condition the detection limit is 8×10-8 mol/Land the linear range of 1.1×10-7～5×10-5 mol/Lis exhibited. The polarographic character of DTT is investigated, the polarographic wave resulted from DTT shows the properties of adsorptive catalysis wave. The electrode reaction mechanisms are discussed in the paper and the effect of Cu2+on the polarographic wave is also studied.

Key words: 1,4-Dithiothreitol, Polarographic adsorptive wave, Polarographic properties of mercapto compound

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