高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (4): 464.

• 论文 • 上一篇    下一篇

筛选抗癌药物的荧光实验法

章开诚, 陈建华, 李志良   

  1. 湖南大学化学化工系, 410082
  • 收稿日期:1989-10-23 出版日期:1991-04-24 发布日期:1991-04-24
  • 通讯作者: 章开诚

Fluorescence Method for Screening Anticancer Drugs

Zhang Kai-cheng, Chen Jiang-hua, Li Zhi-liang   

  1. Department of Chemistry and Chemical Engineering, Hman University, Changsha, 410082
  • Received:1989-10-23 Online:1991-04-24 Published:1991-04-24

摘要: 筛选抗癌药物常用的体外肿瘤系统方法有甲烯蓝法、染色法、细胞呼吸法等,这些方法需要取肿瘤细胞进行实验,时间较长。顺铂是很好的抗癌药物,Sohell等用DNA-溴化乙啶(EthBr)荧光体系研究了铂络合物与DNA的作用,认为该体系可以半定量地说明铂络合物的抗癌性。本文研究发现一些络合物-DNA-EthBr体系的荧光大小与络合物的抗癌性有关,可用荧光法筛选抗癌药物。其可靠性远比甲烯蓝法高,方法简单、快速,且假阳性少。

关键词: 抗癌药物, 荧光法, 络合物

Abstract: Anew method for screening anticancer drugs is proposed. Ethidium bromide(EthBr),a fluorescent dye, can insert into the double helix chains of DNA and increase the intensity of fluorescence. So do some drugs. When this drug is added to the EthBr-DNA fluorescent system, the fluorescence intensity will be decreased. We find that the drug which van decrease 50% in fluorescent intensity of EthBr-DNA system can act with tumour. The fluorescence method is in accordance with the test of S-180 in mice and has more reliability than the methylene blue test.

Key words: Anticancer drugs, Fluorescence method, Complex

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