高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (3): 423.

• 论文 • 上一篇    

木瓜蛋白酶的固定化研究

隋德新, 姜涌明, 赵国骏, 施伟, 史永昶, 严锦文, 姜嘉娴   

  1. 江苏农学院生物化学教研室, 225001
  • 收稿日期:1989-12-16 出版日期:1991-03-24 发布日期:1991-03-24
  • 通讯作者: 隋德新

Studies on Immobilization of Papain

Shui De-xin, Jiang Yong-ming, Zhao Guo-jun, Shi Wei, Shi Yong-chang, Yan Jin-wen, Jiang Jia-xian   

  1. Teaching and Research Section of Biochemistry, Jiangsu Agricultural College, Yangzhou, 225001
  • Received:1989-12-16 Online:1991-03-24 Published:1991-03-24

PDF (PC)

被引次数

17

摘要： 木瓜蛋白酶具有广谱的蛋白水解酶活性,广泛地应用于啤酒、饮料的澄清,肉类嫩化和复配消化药物等方面。近年来,为了扩大该酶在啤洒澄清上的应用,一些研究者致力于探索该酶的固定化研究,以提高该酶的利用效率,降低生产成本。

关键词: 木瓜蛋白酶, 脱乙酰壳多糖, 载体, 固定化, 半衰期

Abstract: This is a report of some results on papain immobilized on chitosan with glutaraldehyde. The effects of the proportion of papain to chitosan, the concentration of glutaraldehyde, temperature, pHvalue and other factors on immobilization of the enzyme were investigated. Characteristic of the immobilized papain was also studied. The results show that the optimum temperature for immobilizing pa-pain is 70℃ 5 the optimum pH is 8. 0; the half-life (t1/2) for the immobilized papain is 30 days; the activity recoverv for the immobilized oaoain is 47%.

Key words: Papain, Chitosan, Carrier, Immobilization, Half-life

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