高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (5): 534.

• 研究简报 • 上一篇    下一篇

柱前螯合反应-高效液相色谱法测定超痕量Fe(Ⅱ),Co(Ⅱ),Fe(Ⅲ)

赵毅, 傅承光   

  1. 河北大学理化分析研究中心
  • 收稿日期:1987-08-12 出版日期:1989-05-24 发布日期:1989-05-24
  • 通讯作者: 傅承光

Precolumn Chelation High Performance Liquid Chromatography Determination of Super- Trace Fe(Ⅱ),Co(Ⅱ ),Fe( Ⅲ)

Zhao Yi, Fu Chengguang   

  1. Research Centre of Physiral and Chemical Analysis, Heber University, Baoding
  • Received:1987-08-12 Online:1989-05-24 Published:1989-05-24

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被引次数

5

摘要： 以吡啶偶氮类螯合剂为配体同时测定多种金属离子的高效液相色谱法研究虽有报道[1～3],但还未见应用4-(5-氯-2-吡啶偶氮)-1,3-二胺基苯(5-Cl-PADAB)为配体与重金属螯合并进行高效液相色谱分离的文献。本文试图用反相分离方式研究Fe(Ⅱ)、Co(Ⅱ)、Fe(Ⅲ)与5-Cl-PADAB螯合物的色谱保留行为并拟建立分离检测方法。研究结果表明,此方法能同时测定Fe(Ⅱ)、Co(Ⅱ)、Fe(Ⅲ),3种离子的检出限分别为4×10-11、4×10-11和2×10-11g。该方法可用于矿样和天然水样分析,尤适于Fe(Ⅱ)、Fe(Ⅲ)的价态分析。

关键词: 高效液相色谱, 柱前螯合, 分离和测定

Abstract: This paper describes the separation of Fe( Ⅱ ),Co(Ⅱ ) and Fe( Ⅲ ) chelates with 5-C1-PADABon C18 bonded stationary phase by RP-Liquid Chro-matography with methanol-water as mobile phase. Heavy-elements such as Fe(Ⅱ), Co(Ⅱ ) and Fe( Ⅲ ) were determined by this chromatographic system. The retention of the chelates depends on central metal ions, mcthanol-water ratio, pHvalue of the mobile plase and concentration of NaH2PO4 in mobile phase. The chelates of Fe( Ⅱ ),Co(Ⅱ ),Fe(Ⅲ) at part billion levels were separated within 10 min by using 5μm Nucleosil C18 column(250 × 4mm I.D) with methanol(65)-water(35 containning 2.0g/L NaH2PO4) as the mobile phase with pH 2.5. The detection limits of Fe( Ⅱ ), Co( Ⅱ) and Fe( Ⅲ) are 4 × 10-11g,4 × 10-11g and 2×10-11g respectively.

Key words: High performance liquid chromatography, Precolumn chelation, Separation and determination

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