高等学校化学学报

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电导调控的双极电极电化学发光法检测尿素

杨碧芳1,郭晓戈1,戴小辉2,杨伟强1,倪建聪1   

  1. 1. 闽南师范大学 化学化工与环境学院 2. 福建省漳州市产品质量检验所

  • 收稿日期:2025-04-03 修回日期:2025-05-30 出版日期:2025-06-23 发布日期:2025-06-23
  • 通讯作者: 倪建聪 E-mail:nijiancong@foxmail.com
  • 基金资助:
    福建省市场监督管理局科技项目(No. FJMS2023065)资助

Detection of Urea by Conductivity - Regulated Bipolar Electrode Electrochemiluminescence

YANG Bifang1, GUO Xiaoge1, DAI Xiaohui2, YANG Weiqiang1, NI Jiancong1   

  1. 1. Minnan Normal University Chemistry, Chemical Engineering and Environment 2. Zhangzhou Product Quality Inspection Institute
  • Received:2025-04-03 Revised:2025-05-30 Online:2025-06-23 Published:2025-06-23
  • Contact: NI Jiancong E-mail:nijiancong@foxmail.com
  • Supported by:
    Supported by the Science and Technology Project of Fujian Provincial Administration of Market Supervision (No. FJMS2023065)

摘要: 本研究构建了一种基于溶液电导调控的双极电极电化学发光(BPE - ECL)传感平台,用于尿素的定量检测. 在图案化的双极电极(BPE)上构建独立的传感检测池与信号收集池,采用钌联吡啶/三正丙胺[Ru(bpy)32+/TPrA]作为的电化学发光(ECL)试剂. 当传感池中存在不同浓度的尿素时,脲酶特异性催化分解尿素分子,产生导电离子促使溶液电导增大,基于BPE的电荷平衡机制,信号池中ECL响应强度随之增强. 尿素浓度越高,ECL信号强度越大,据此建立基于电导调控的BPE-ECL平台用于尿素的检测. 在最优条件下,该传感平台对尿素在10 nM – 1 mM范围内呈现良好的线性关系,检出限为0.19 nM (S/N = 3). 将传感进一步用于人尿液样品中的加标回收检测,表现出良好的准确性和抗干扰能力. 基于溶液电导调控的传感机制不仅无需加入外源电活性指示剂,而且传感-信号分离的模式能有效避免了复杂生物样本与信号反应交叉的干扰.

关键词: 双极电极, 电化学发光, 电导调控, 传感-信号分离, 尿素

Abstract: In this study, a bipolar - electrode electrochemiluminescence (BPE-ECL) sensing platform based on the regulation of conductivity was constructed for the detection of urea. An independent sensing cell and a reporting cell were constructed on the patterned BPE, and ruthenium bipyridine / tri-n-propylamine [Ru(bpy)32+/TPrA] was used as the ECL – signal resource. When urea at different concentrations is present in the sensing cell, urease specifically catalyzes the decomposition of urea, generating ions that promote an increase in the conductivity. Based on the charge balance of BPE, the intensity of ECL in the reporting cell increases. The higher the concentration of urea, the greater the intensity of ECL. Therefore, a BPE-ECL platform based on regulation of conductivity was established for the detection of urea. Under optimal conditions, this sensing platform exhibits a good linear relationship for urea within the range of 10 nM – 1 mM, and the limit of detection is 0.19 nM (S/N = 3). The sensing platform was further applied to the spiked recovery detection in human urine samples, showing good accuracy and anti - interference ability. The sensing strategy based on the regulation of conductivity not only eliminates the need for the addition of exogenous electroactive indicators, but also effectively avoids the interference of complex biological samples with the signal reaction through the sensing - signal separation of BPE.

Key words: Bipolar electrode, Electrochemiluminescence , ; Conductivity regulation, Sensing-signal separation, Urea

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