高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

Sr2+/NO/OGP协同提高钛表面TiO2纳米管的成骨性能研究

潘长江1,马文富2,丁平云1,张秋阳2,邓林红1   

  1. 1. 常州大学，生物医学工程与健康科学研究院
    2. 淮阴工学院，江苏省医用材料及高端医疗器械研究工程中心


  • 收稿日期:2024-11-29 修回日期:2025-03-16 出版日期:2025-03-18 发布日期:2025-03-18
  • 通讯作者: 潘长江 E-mail:pcj@cczu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号：31870952)和江苏省自然科学基金(批准号：BK20241941)资助

Synergistically improving osteogenic properties of TiO2 nanotubes on titanium surface by Sr2+/NO/OGP

PAN Changjiang1, MA Wenfu2, DING Pingyun1, ZHANG Qiuyang2, DENG Linhong1   

  1. 1. Institute of Biomedical Engineering and Health Science, Changzhou University
    2. Jiangsu Provincial Engineering Research Center for Biomaterials and Advanced Medical Devices, Huaiyin Institute of Technology

  • Received:2024-11-29 Revised:2025-03-16 Online:2025-03-18 Published:2025-03-18
  • Contact: Changjiang Pan E-mail:pcj@cczu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. 31870952) and the Natural Science Foundation of Jiangsu Province (No. BK20241941)

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摘要： 本文首先采用阳极氧化和水热处理技术在钛表面原位制备装载Sr2+的TiO2纳米管阵列，然后采用电泳沉积的方法在纳米管表面制备羧甲基壳聚糖(CMCS)涂层，最后依次在纳米管中装载NO释放分子(N-亚硝基-N-苯基羟胺铵盐)和成骨生长肽(OGP)，通过Sr2+、NO气体分子以及OGP协同促进成骨细胞的粘附、生长和功能表达. 研究结果表明，功能化的纳米管阵列不仅可以诱导羟基磷灰石(HA)的仿生沉积，还可以持续释放Sr2+和NO气体信号分子，显著促进成骨细胞的粘附、增殖以及碱性磷酸酶(ALP)、骨钙素(OCN）和Runt相关转录因子2(RUNX2)的表达. 在装载OGP后，成骨细胞的粘附生长和功能表达进一步提高. 因此，本文的表面改性策略可用于在钛合金表面构建具有优异生物相容性的生物活性涂层，从而提高钛基骨替代材料的骨整合性能.

关键词:  , 钛；成骨细胞；NO气体分子

Abstract: In this study, anodization and hydrothermal treatment techniques were first employed to prepare Sr2+-loaded TiO2 nanotube arrays on the titanium surface. Then, carboxymethyl chitosan (CMCS) coating was fabricated on the nanotube surface by electrophoretic deposition. Finally, NO-releasing molecules (N-Nitroso-N-phenylhydroxylamine ammonium salt, Cupferron) and osteogenic growth peptides (OGP) were sequentially loaded into the nanotubes, achieving synergistically promoting osteoblast adhesion, proliferation, and functional expression by Sr2+, NO gas molecule, and OGP. The results indicated that the functionalized nanotube arrays could not only induce biomimetic deposition of hydroxyapatite (HA), but also continuously release Sr2+ and NO gas signaling molecules, significantly promoting the adhesion and growth of osteoblasts, as well as the expressions of alkaline phosphatase (ALP), osteocalcin (OCN), and Runt related transcription factor 2 (RUNX2). After loading OGP, the osteoblast adhesion, growth, and functional expression were further enhanced. Therefore, the surface modification strategy of the present study can be used to construct the bioactive coating with excellent biocompatibility on titanium alloy surfaces to improve the bone integration ability of titanium-based bone-substituted materials.

Key words: Titanium, Osteoblast, NO gas molecule

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