高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (9): 1897-1903.doi: 10.7503/cjcu20190167

• 有机化学 • 上一篇    下一篇

开口箭化学成分及抗肿瘤活性

肖艳华1,*(),张广杰2,宗良1,刘国宏1,任丽君1,董俊兴2,*()   

  1. 1. 陆军防化学院, 北京 102205
    2. 军事科学院军事医学研究院, 北京 100850
  • 收稿日期:2019-03-19 出版日期:2019-09-10 发布日期:2019-09-09
  • 通讯作者: 肖艳华,董俊兴 E-mail:yanhuaxiao@163.com;djx931314@163.com
  • 基金资助:
    国家科技重大专项(2013ZX09J13101-02B)

Chemical Constituents and Antitumor Activity of Tupistra chinensis

XIAO Yanhua1,*(),ZHANG Guangjie2,ZONG Liang1,LIU Guohong1,REN Lijun1,DONG Junxing2,*()   

  1. 1. Institute of NBC Defence, Beijing 102205, China
    2. Academy of Military Medical Sciences, Beijing 100850, China
  • Received:2019-03-19 Online:2019-09-10 Published:2019-09-09
  • Contact: XIAO Yanhua,DONG Junxing E-mail:yanhuaxiao@163.com;djx931314@163.com
  • Supported by:
    Supported by the National Science and Technology Major Project of China(2013ZX09J13101-02B)

摘要:

从开口箭新鲜根茎中分离得到8个化合物, 通过理化性质分析、 质谱、 红外光谱及核磁共振波谱检测等方法鉴定了其结构, 分别为开口箭皂苷J(1)、 (25S)-26-O-β-D-吡喃葡萄糖基呋甾-1β,3β,22α,26-四羟基-3-O-β-D-吡喃葡萄糖苷(2)、 洋地黄毒苷元-3-O-α-L-吡喃岩藻糖苷(3)、 弯蕊皂苷元B(4)、 异罗斯考皂苷元(5)、 罗斯考皂苷元(6)、 香豌豆酚(7)及(+)-儿茶素(8). 其中, 化合物1为新化合物, 化合物3, 4, 7和8为首次从该属植物中分离得到. 在抗人结肠癌细胞LoVo和胃癌细胞BGC-823活性评价中, 化合物5和6表现出较强的抗肿瘤活性, 半抑制浓度(IC50)值分别达到0.532和0.757 μmol/L.

关键词: 开口箭, 化学成分, 抗肿瘤活性

Abstract:

Tupistra chinensis(Liliaceae), mainly distributed in southwestern China, is a rich source of steroidal sapogenins, saponins and flavans. These components exhibit diverse biological activities such as anti-inflammatory, anti-fungal and anti-tumor properties. As a part of a continuing study for the discovery of novel cytotoxic agents, eight compounds were isolated from the fresh rhizomes of Tupistra chinensis, and their structures were established on the basis of chemical methods and spectral data(MS, IR, NMR), including tupistroside J(1), (25S)-26-O-β-D-glucopyranosyl-furost-1β,3β,22α,26-tetraol-3-O-β-D-glucoside(2), digitoxigenin-3-O-α-L-fucopyranose(3), watti-genin B(4), epiruscogenin(5), epiruscogenin(6), orobol(7), (+)-catechin(8). Compound 1 was a new compound, and compounds 3, 4, 7 and 8 were isolated from Tupistra Ker-Gawl for the first time. All compounds were tested in vitro for their cytotoxic activities against the Human LoVo and BGC-823 cell lines, and compounds 5 and 6 were found to possess significant cytotoxicity against the tested tumor cell lines with half maximal inhibitory concentration(IC50) values of 0.532 and 0.757 μmol/L, respectively.

Key words: Tupistra chinensis baker, Chemical constituent, Antitumor activity

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