高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (7): 1390-1396.doi: 10.7503/cjcu20180812

• 分析化学 • 上一篇    下一篇

稀有原人参二醇型皂苷的人肠道菌群生物转化

韩铭鑫, 李方彤, 张琰, 戴雨霖, 郑飞(), 越皓()   

  1. 长春中医药大学, 吉林省人参科学研究院, 长春 130117
  • 收稿日期:2018-12-03 出版日期:2019-07-10 发布日期:2019-07-12
  • 作者简介:郑 飞, 女, 助理研究员, 主要从事保健食品开发方面的研究. E-mail: zhengfei@ccucm.edu.cn;越 皓, 男, 博士, 研究员, 博士生导师, 主要从事中药化学分析方面的研究. E-mail: yuehao@sohu.com
  • 基金资助:
    国家重点研发计划项目(批准号: 2017YFC1702105)、 吉林省教育厅“十三五”科学技术项目(批准号: JJKH20181270KJ)和吉林省科技发展计划项目(批准号: 20180311019YY)资助.

Biotransformation of Rare Protopanaxadiol Saponin by Human Intestinal Microflora

HAN Mingxin, LI Fangtong, ZHANG Yan, DAI Yulin, ZHENG Fei*(), YUE Hao*()   

  1. Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China
  • Received:2018-12-03 Online:2019-07-10 Published:2019-07-12
  • Contact: ZHENG Fei,YUE Hao E-mail:zhengfei@ccucm.edu.cn;yuehao@sohu.com
  • Supported by:
    † Supported by the National Key R&D Program of China(No.2017YFC1702105), the 13th Five?Year Science and Technology Development Plan Project of Jilin Province, China(No.JJKH20181270KJ) and the Science and Technology Development Project of Jilin Province, China(No.20180311019YY).

摘要:

利用高分离度液相色谱-四极杆飞行时间质谱(RRLC-Q-TOF MS)和超高效液相色谱-三重四极杆质谱(UPLC-QQQ MS)定性定量分析了稀有原人参二醇型皂苷Rd, F2, Rg3, CK和Rh2在离体人肠道菌群中的生物转化过程. 并将上述二醇型皂苷与人肠道菌群在体外厌氧, 37 ℃下共温孵育, 采用电喷雾质谱在负离子模式进行检测, 鉴定代谢产物, 监测其含量变化, 拟合代谢路径. 结果表明, 人参皂苷Rd主要被代谢为F2, Rg3, CK, Rh2和PPD; 人参皂苷F2主要被代谢为CK和PPD; 人参皂苷Rg3主要被代谢为Rh2和PPD; 人参皂苷CK和Rh2主要被代谢为PPD. 在离体条件下, 人参皂苷Rd, F2和Rg3会被肠道菌群完全转化为其代谢产物, 而人参皂苷CK和Rh2则不能被肠道菌群完全转化为其代谢产物. 原人参二醇型皂苷在人肠道菌群中的主要转化为脱糖基反应, 单糖苷和苷元是稀有原人参二醇型皂苷在人体内发挥药效的物质基础.

关键词: 稀有原人参二醇型皂苷, 人肠道菌群, 生物转化, 液相色谱-质谱联用

Abstract:

The rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry(RRLC-Q-TOF MS) method and ultra high performance liquid chromatography-triple quadruple mass spectrometry(UPLC-QQQ MS) method were applied for qualitative and quantitative analyzing the biotransformation process of rare protopanaxadiol saponin Rd, Rg3, F2, CK and Rh2 under intestinal micro flora in vitro. The rare protopanaxadiol saponin Rd, F2, Rg3, CK, Rh2 and intestinal micro flora were cultured in vitro at 37 ℃ under anaerobic condition. The mass spectrometrics detection with electrospray ionization under negative ion mode was applied for identifying the metabolites, monitoring content variation, and matching metabolic pathways. The results show ginsenoside Rd is mainly metabolized into five products of F2, Rg3, CK, Rh2 and PPD. Ginsenoside F2 is mainly metabolized into two products of CK and PPD. Ginsenoside Rg3 is mainly metabolized into two products CK and PPD. Ginsenosides CK and Rh2 are both mainly metabolized into one product PPD. Ginsenosides Rd, F2 and Rg3 are metabolized completely into metabolic products, but ginsenosides CK and Rh2 are not, under human intestinal micro flora condition in vitro. The results also indicated that deglycosylation reaction was the major metabolic pathway of rare protopanaxadiol saponin under the human intestinal microflora condition. And that monoglycosides and aglycone may be the material basis for rare protopanaxadiol saponin to exert its efficacy in human body.

Key words: Rare protopanaxadiol saponin, Human intestinal micro flora, Biotransformation, Liquid chromatography-mass spectrometry

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