HA/GCPU复合多孔支架的可控制备及细胞相容性

doi:10.7503/cjcu20170749

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (7): 1580.doi: 10.7503/cjcu20170749

HA/GCPU复合多孔支架的可控制备及细胞相容性

杜晶晶¹, 黄棣^1,²(), 魏延¹, 连小洁¹, 胡银春¹, 王楷群¹, 刘阳², 陈维毅^1,²

1. 太原理工大学力学学院生物医学工程系, 纳米生物材料与再生医学研究中心,太原 030024
2. 应用力学与生物医学工程研究所, 材料强度与结构冲击山西省重点实验室, 太原 030024

收稿日期:2017-11-20 出版日期:2018-07-10 发布日期:2018-06-21
作者简介:联系人简介: 黄棣, 男, 博士, 副教授, 主要从事纳米生物材料研究. E-mail: huangjw2067@163.com
基金资助:
国家自然科学基金(批准号: 11502158, 51503140, 11632013, 11502156)和山西省青年科技基金(批准号: 2015021195)资助.

Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based olyurethane Porous Scaffolds^†

DU Jingjing¹, HUANG Di^1,^2,^*(), WEI Yan¹, LIAN Xiaojie¹, HU Yinchun¹, WANG Kaiqun¹, LIU Yang², CHEN Weiyi^1,²

1. Research Center for Nano-biomaterials & Regenerative Medicine, Department of Biomedical Engineering,College of Mechanics, Taiyuan 030024, China
2. Institute of Applied Mechanics & Biomedical Engineering, Shanxi Key Labratory of aterials Strength & Structrual Impact, Taiyuan University of Technology, Taiyuan 030024, China

Received:2017-11-20 Online:2018-07-10 Published:2018-06-21
Contact: HUANG Di E-mail:huangjw2067@163.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.11502158, 51503140, 11632013, 11502156) and the Natural Science Foundation of Shanxi Province, China(No. 2015021195).

摘要/Abstract

摘要：

以脂肪族异佛尔酮二异氰酸酯(IPDI)作为硬段、蓖麻油甘油酯(GCO)作为软段, 通过原位聚合法制备了羟基磷灰石/蓖麻油甘油酯基聚氨酯(HA/GCPU)复合多孔支架. 利用红外光谱和扫描电子显微镜对复合支架进行表征, 并测试其力学性能及孔隙率. 研究结果表明, HA/GCPU复合多孔支架的孔隙率和抗压强度依赖羟基磷灰石的含量, 并具有明显的可控性. HA质量分数分别为0, 20%和40%时, HA/GCPU复合多孔支架孔隙率分别为(61±3)%, (68±2)%和(57±3)%, 抗压强度分别为(605±61), (2125±58)和(4588±260) kPa, 可见HA质量分数为20%时, HA/GCPU复合多孔支架具有与松质骨较为匹配的孔隙率和抗压强度. 将MG63细胞与多孔支架共培养, 通过倒置显微镜和扫描电子显微镜观察, 用噻唑蓝(MTT)法表征HA/GCPU复合多孔支架的细胞相容性, 结果表明, HA/GCPU复合多孔支架表面细胞黏附, 生长良好, 无细胞毒性, 在骨组织工程领域具有一定的应用前景.

关键词: 蓖麻油, 甘油酯聚氨酯, 羟基磷灰石, 多孔支架, 细胞相容性

Abstract:

Castor oil was transesterified with glycerol, the glyceride of castor oil(GCO) copolymerized with isophorone diisocyanate(IPDI) to generate polyurethane(PU). The porous scaffolds of hydroxyapatite/glyceride based polyurethae(HA/GCPU) were prepared by in situ polymerization. The GCPU and HA/GCPU porous scaffolds were characterized by Fourier transforw infrared spectroscopy(FTIR) and scanning electron microscopy(SEM). The mechanical property and porosity were tested. The results showed that the porosity of GCPU, HA/GCPU composite with 20% HA and HA/GCPU composite with 40% HA were (61±3)%, (68±2)% and (57±3)%, respectively. The compressive strength of above three porous scaffolds were 605±61, 2125±58 and (4588±260) kPa, respectively. To further evaluate the cytocompatibility of porous scaffolds, the MG63 cells were co-cultured on scaffolds, and light microscopy observation, SEM observation, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium-bromide(MTT) assay were carried out. The results demonstrated that both pure GCPU and HA/GCPU scaffolds had no cytotoxicity. It is believed that HA/GCPU composite porous scaffolds have the potential to be applied in bone regeneration.

Key words: Castor oil, Glyceride polyurethane, Hydroxyapatite, Porous scaffolds, Cytocompatibility

中图分类号:

O631
O646

TrendMD:

杜晶晶, 黄棣, 魏延, 连小洁, 胡银春, 王楷群, 刘阳, 陈维毅. HA/GCPU复合多孔支架的可控制备及细胞相容性. 高等学校化学学报, 2018, 39(7): 1580.

DU Jingjing, HUANG Di, WEI Yan, LIAN Xiaojie, HU Yinchun, WANG Kaiqun, LIU Yang, CHEN Weiyi. Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based olyurethane Porous Scaffolds^†. Chem. J. Chinese Universities, 2018, 39(7): 1580.

图/表 8

Fig.1 FTIR spectra of glycerol(a), castor oil(b) and GCO(c)

Fig.2 FTIR spectra of IPDI(a), GCPU(b), 20% HA/GCPU(c), 40% HA/GCPU(d) and HA(e)

Fig.3 SEM images of HA microspheres(A) and enlarged photo(B)

Fig.4 SEM images(A1—C1) and corresponding EDS map scanning analysis(A2, A3, B2, B3, C2, C3) of GCPU(A1—A3), 20% HA/GCPU(B1—B3) and 40% HA/GCPU(C1—C3)^(A2) C; (A3) O; (B2) Ca; (B3) P; (C2) Ca; (C3) P. Insets of (A1, B1, C1) are EDS spectra.

Table 1 Porosity and compressive strength of HA/GCPU scaffolds

Sample	w(HA)(%)	Porosity(%)	Compressive strength/kPa
1	0	61±3	605±61
2	20	68±2	2125±58
3	40	57±3	4588±260

Fig.5 Inverted microscopy photographs of MG63 cells cultured on GCPU(A1—A3), 20% HA/GCPU(B1—B3), 40% HA/GCPU(C1—C3) for 1 d(A1—C1), 4 d(A2—C2) and 11 d(A3—C3)

Fig.6 MTT assay for proliferation of MG63 cells cultured with scaffolds^ *p<0.05; **p<0.01.

Fig.7 SEM images of MG63 cells cultured on GCPU(A1, A2), 20% HA/GCPU(B1, B2), 40% HA/GCPU(C1, C2) for 7 d^ (A2)—(C2) higher magnification images of white rectangles in (A1)—(C1).

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HA/GCPU复合多孔支架的可控制备及细胞相容性

Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based olyurethane Porous Scaffolds^†

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HA/GCPU复合多孔支架的可控制备及细胞相容性

Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based olyurethane Porous Scaffolds†

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Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based olyurethane Porous Scaffolds^†