高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (9): 1596-1601.doi: 10.7503/cjcu20160884

• 有机化学 • 上一篇    下一篇

Slc11a2第六跨膜区在十二烷基磷酰胆碱胶束中的结构

肖淑艳1, 李菲2, 董忠平1()   

  1. 1. 内蒙古科技大学材料与冶金学院, 包头 014010
    2. 吉林大学超分子结构与材料国家重点实验室, 长春 130012
  • 收稿日期:2016-12-07 出版日期:2017-09-10 发布日期:2017-08-25
  • 作者简介:联系人简介: 董忠平, 男, 博士, 讲师, 主要从事蛋白质谱学研究. E-mail: dzp05291128@163.com
  • 基金资助:
    国家自然科学基金(批准号: 21663017)、 内蒙古自治区自然科学基金(批准号: 2015BS0205)、 内蒙古自治区草原英才基金(批准号: CYYC2013173)和内蒙古科技大学创新基金(批准号: 2014QDL017)资助

Solution Structure of Slc11a2-TM6 in Dodecylphosphocholine Micelles

XIAO Shuyan1, LI Fei2, DONG Zhongping1,*()   

  1. 1. School of Materials and Metallurgy, Inner Mongolia University of Science and Technology, Baotou 014010, China
    2. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  • Received:2016-12-07 Online:2017-09-10 Published:2017-08-25
  • Contact: DONG Zhongping E-mail:dzp05291128@163.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21663017), the Natural Science Foundation of Inner Mongolia, China(No.2015BS0205), the Grassland Talent Foundation of Inner Mongolia, China(No.CYYC2013173) and the Inner Mongolia University of Science and Technology Innovation Fund, China(No.2014QDL017)

摘要:

采用圆二色谱和核磁共振波谱研究了Slc11a2第六跨膜区在十二烷基磷酰胆碱(DPC)膜模拟环境中的结构和定位. 结果表明, Slc11a2第六跨膜区在DPC胶束中N端和C端各形成一小段α螺旋, 两段螺旋中间通过高度灵活的区域连接, 整个肽插入到DPC胶束中. H267A突变使螺旋长度变长, H272A突变使螺旋长度略有变短, 突变后结构的变化可能和蛋白功能缺失相关.

关键词: Slc11a2第六跨膜区, 核磁共振, 三维结构

Abstract:

The structure of isolated peptides from TM6 of Slc11a2 in dodecylphosphocholine(DPC) micelles was investigated using circular dichroism(CD) and nuclear magnetic resonance(NMR) spectroscopy. The results reveal that TM6 of Slc11a2 forms short α-helix in both N- and C-terminal and the two α-helixes are connected by a highly flexible region, the whole peptide is embedded in DPC micelles. H267A mutation makes the helix become longer, while H272A mutation make the helix become slightly shorter.

Key words: Slc11a2-TM6, Nuclear magnetic resonance, 3D structure(Ed.: S, Z, M)

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