海藻酸钠和壳聚糖静电复合弹性支架的制备和表征

doi:10.7503/cjcu20141094

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (4): 758.doi: 10.7503/cjcu20141094

海藻酸钠和壳聚糖静电复合弹性支架的制备和表征

张伟骏, 张坤玺, 李贵飞, 张丹青, 尹静波()

上海大学材料学院高分子材料系, 上海 201800

收稿日期:2014-12-15 出版日期:2015-04-10 发布日期:2015-03-27
作者简介:联系人简介: 尹静波, 女, 博士, 教授, 主要从事生物医用和生态环境高分子材料研究. E-mail: jbyin@oa.shu.edu.cn
基金资助:
国家自然科学基金(批准号: 51373094, 51173101)和上海市科委基础研究重点项目(批准号: 11JC1404200)资助

Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds^†

ZHANG Weijun, ZHANG Kunxi, LI Guifei, ZHANG Danqing, YIN Jingbo^*()

Department of Polymer Materials, School of Materials Science and Engineering,Shanghai University, Shanghai 201800, China

Received:2014-12-15 Online:2015-04-10 Published:2015-03-27
Contact: YIN Jingbo E-mail:jbyin@oa.shu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.51373094, 51173101) and the Science and Technology Commission of Shanghai Municipality, China(No.11JC1404200)

摘要/Abstract

摘要：

通过静电作用和相分离技术制备海藻酸钠/壳聚糖静电复合弹性支架, 研究了冷冻温度和固含量对支架材料孔径的影响及组分比对材料力学性能、亲水性、降解性能和生物相容性的影响. 固含量为2%(质量分数)及冷冻温度为-24 ℃时, 支架孔径为110~170 μm, 并且亲水性良好, 平衡溶胀度大于1400%. 改变固含量和组分比可调控材料的力学性能; 循环力学测试表明, 湿态支架具有良好的弹性和一定的耐疲劳性; 降解速率可由组分比调控; 兔脂肪干细胞(rASCs)在支架上的培养结果表明, 羧基和氨基摩尔比为2:1和1:1时细胞以聚集体存在; 羧基和氨基摩尔比为1:2时细胞黏附于支架上, 实现细胞黏附/聚集体的调控.

关键词: 组织工程支架, 海藻酸钠, 壳聚糖

Abstract:

Elastic scaffolds composed of sodium alginate(SA) and chitosan(CS) were fabricated via electrostatic interaction between —COOH, —NH₂ groups and phase separation technique. This study presented the influence of freezing temperature, solid content on pore size and molar ratio on mechanical strength, hydrophilicity, biodegradability and biocompatibility. The results showed that average pore size of alginate/chitosan scaffolds fabricated from 2%(mass fraction) solid content and at a freezing temperature of -24 ℃ was between 110 and 170 μm. High swelling ratio of more than 1400% showed the excellent hydrophilic performance of these scaffolds. Degradation of the alginate/chitosan scaffolds could be modified by varying the molar ratio(—COOH/—NH₂). The mechanical strength of the scaffolds could be controlled by different molar ratios and solid contents. The scaffolds exhibited good elastic behavior and fatigue durability by cyclic mechanical test in wet state. In vitro culture of rabbit adipose-derived stem cells(rASCs) indicated that aggregated rASCs were formed in the scaffolds when the molar ratios were 2:1 and 1:1, while adherent rASCs were found in the scaffolds with molar ratio of 1:2. Hence cellular adhesion behavior can be regulated by varying scaffold component.

Key words: Tissue engineering scaffold, Alginate, Chitosan

中图分类号:

O636

TrendMD:

张伟骏, 张坤玺, 李贵飞, 张丹青, 尹静波. 海藻酸钠和壳聚糖静电复合弹性支架的制备和表征. 高等学校化学学报, 2015, 36(4): 758.

ZHANG Weijun, ZHANG Kunxi, LI Guifei, ZHANG Danqing, YIN Jingbo. Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds^†. Chem. J. Chinese Universities, 2015, 36(4): 758.

图/表 9

Fig.1 Fabricating process SA/CS scaffolds

Fig.2 FTIR spectra of scaffolds a. SA; b. CS; c. SA/CS; d. SA/CS crosslinked with Ca2+.

Fig.3 SEM images of SA/CS scaffolds (A) -7 ℃; (B) -24 ℃; (C) -80 ℃, SA/CS molar ratio: 1:1, solid content: 2%; (D) solid content: 1%; (E) solid content: 2%; (F) solid content: 3%, SA/CS molar ratio: 1:1, freezing temperature: -24 ℃.

Fig.4 Pore size distributions with different temperature(A) and different solid content(B) and porosity of SA/CS scaffolds(molar ratio 1:1) with different solid contents(C)

Fig.5 Elasticity presentation(A), compression modulus of SA/CS scaffolds(B, C) and cyclic mechanical test of SA/CS scaffolds(D—F) (B) SA/CS molar ratio 1:1; (C) solid content 2%; molar ratio of SA/CS: (D) 2:1; (E) 1:1; (F) 1:2.

Fig.6 Contact angle and swelling ratio of SA/CS scaffolds (A, C) Solid content: 2%; (B) SA/CS molar ratio: 1:1.

Fig.7 Degradation of SA/CS scaffolds(solid content 2%) with different molar ratios Molar ratio of SA/CS: a. 1:2; b. 1:1; c. 2:1.

Fig.8 Cell adhesion on SA/CS scaffolds for 7 d(solid content 2%) Molar ratio of SA/CS: (A) 2:1; (B) 1:1; (C, D) 1:2; (D) high magnification of (C).

Fig.9 DNA content of rASCs in SA/CS scaffolds for 7 d determined by Hoechst 33258 DNA assay(solid content 2%)

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海藻酸钠和壳聚糖静电复合弹性支架的制备和表征

Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds^†

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海藻酸钠和壳聚糖静电复合弹性支架的制备和表征

Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds†

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Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds^†