两亲性多肽自组装及其应用

doi:10.7503/cjcu20140859

摘要/Abstract

摘要：

两亲性多肽分子具有类似天然磷脂分子的两亲特性、丰富的分子结构、独特新颖的组装体结构以及特殊的生物学功能, 是多肽自组装研究的热点领域. 本文总结了近年来关于两亲性多肽自组装研究及应用的进展, 介绍了几种常见的两亲性多肽, 并进一步阐述其分子结构特征、组装行为和机理、组装体结构和功能以及在纳米技术和生物医学领域中的应用.

关键词: 两亲性多肽, 自组装, 形貌, 纳米结构, 生物功能材料

Abstract:

Due to good biocompatibility, straight forward chemical modification and inherent molecular recognition, peptides have been very attractive biological building blocks for self-assembly. Amphiphilic peptides are of special interesting with the advantages of abundant molecular structures, unique structures of assemblies and special biological functions. In this work, the recent progress in the study and application of the self-assembly of amphiphilic peptides was reviewed. Several kinds of amphiphilic peptides were introduced. Moreover, the molecular structures of amphiphilic peptides, the behavior and mechanism of self-assembly, the structure and property of assemblies and their applications in nanotechnology and biomedical field were also demonstrated in detail.

Key words: Amphiphilic peptide, Self-assembly, Morphology, Nano structure, Biological functional material

中图分类号:

O631

TrendMD:

王建勋, 秦四勇, 蔡腾腾, 张先正, 卓仁禧. 两亲性多肽自组装及其应用. 高等学校化学学报, 2015, 36(2): 201.

WANG Jianxun, QIN Siyong, CAI Tengteng, ZHANG Xianzheng, ZHUO Renxi. Self-assembly of Peptide Amphiphiles and Their Applications^†. Chem. J. Chinese Universities, 2015, 36(2): 201.

图/表 8

Fig.1 Schematic illustration of the self-assembly of surfactant-like peptides into nano-structures[4,6] (A) Copyright from American Chemical Society; (B) copyight from the Royal Society of Chemistry.

Fig.2 Structural models of peptide amphiphiles proposed by Kunitake[15](A) and schematic illustration of the self-assembly of PAs[20](B) (A) Copyright from the Royal Society of Chemistry; (B) copyright from Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Fig.3 TEM images of quadruple helix fibers(A), single fibers[26](B), PA before(C) and after(D) photoirradiation[27] and cell survival and morphology of NPCs encapsulated in IKVAV gels[31](E1—E3) (A) Copyright from American Chemical Society; (B) copyright from Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim; (C) copyright from American Association for the Advancement of Science. Cell survival determined by LIVE/DEAD cell assay at 1 d(E1), 7 d(E2) and 22 d(E3).

Fig.4 Schematic depiction of the formation of gold nanoparticle double helices facilitated by the self-assembling peptide(A), TEM images of left-handed gold nanoparticle double helices(B, C) and tomographic 3D reconstruction image of the double helices[35](D) Copyright from American Chemical Society.

Fig.5 Proposed hierarchical self-assembly mechanisms of CPs2(A) and CPs3(B)[48] Copyright from the Royal Society of Chemistry.

Fig.6 Complexes formation, internalization and cellular trafficking process of PA gene delivery[52](A) and schematic illustration of MENPs self-assembly of PA-DOX and the targeted release of DOX in tumor cells[53](B) (A) Copyright from Elsevier B.V; (B) copyright from American Chemical Society.

Fig.7 GG-C7-GG assembled into helical ribbons in pH=8 and GG-C7-GG assembled into tubule structures in pH=3(A) and proposed assembly mechanism[54](B) Copyright from American Chemical Society.

Fig.8 Schemaic representation and TEM images of nanofibril formed by 2C12-Lys-Aβ(12—17)(a), twisted ribbon formed by C12-Aβ(11—17)-C12 at low pH(b), lamellae formed by C12-Aβ(11—17)-C12 at high pH(c)[73](A), TEM image of neutral(C12CCP)n/DNA complexes(B) and pharmocokinetics and biodistribution of various DNA complexes following injection of plasmid into the jugular vein of mice[76](C) Copyright from American Chemical Society.

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