高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (3): 679.doi: 10.7503/cjcu20120838

• 高分子化学 • 上一篇    下一篇

纳米银/二甲基砜/聚乳酸-乙醇酸静电纺丝人工敷料的制备及生物评价

崔巍巍1,2, 刘娅2, 王宗良1, 王昊2, 崔立国1, 章培标1, 陈学思1   

  1. 1. 中国科学院长春应用化学研究所, 生态环境高分子材料重点实验室, 长春 130022;
    2. 吉林大学公共卫生学院, 长春 130021
  • 收稿日期:2012-09-12 出版日期:2013-03-10 发布日期:2013-02-18
  • 通讯作者: 章培标,男,博士,副研究员,主要从事生物高分子材料研究.E-mail:zhangpb@ciac.jl.cn;刘娅,女,教授,博士生导师,主要从事生物安全性评价研究.E-mail:liuya@jlu.edu.cn E-mail:zhangpb@ciac.jl.cn;liuya@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:50973109,51103149)和吉林大学研究生创新基金(批准号:20121111)资助.

Preparation and Biological Evaluation of Electrospun MSM/PLGA Dressing Containing Nano-silver

CUI Wei-Wei1,2, LIU Ya2, WANG Zong-Liang1, WANG Hao2, CUI Li-Guo1, ZHANG Pei-Biao1, CHEN Xue-Si1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Key Laboratary of Polymer Ecomaterials, Changchun 130022, China;
    2. School of Public Health, Jilin University, Changchun 130021, China
  • Received:2012-09-12 Online:2013-03-10 Published:2013-02-18

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被引次数

29

摘要：

采用静电纺丝方法制备了纳米银(n-Ag)/二甲基砜(MSM)/聚乳酸-乙醇酸(PLGA)抗菌人工敷料. 通过场发射扫描电子显微镜(ESEM)和能量离散X射线光谱(EDX)研究敷料的微观结构及表面元素组成. 同时对敷料的力学性能、 吸水性、 细胞相容性及抗菌性进行测试, 结果表明, 该敷料内部纤维呈交叉的网格状结构, 互相连结, 随着n-Ag含量的增加, 纤维的力学强度逐渐增大, 吸水性能逐渐增强. 当n-Ag质量分数达到1%以上时, 敷料对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性大肠杆菌具有良好的抗菌活性. 细胞实验结果表明, 当n-Ag含量在0.01%～10%之间时, 敷料属无毒性或低毒性; 当其含量小于1%时, 有助于细胞的生长和增殖. 因此, 该敷料具有良好的细胞相容性和抗菌性能.

关键词: 静电纺丝, 纳米银, 聚乳酸-乙醇酸, 敷料

Abstract:

Antibacterial dressings containing nano-silver powders(n-Ag)/dimethyl sulfoxide(MSM)/poly(lactide-co-glycolide)(PLGA) were prepared by electrospinning. Field emission scanning electron microscope(ESEM) and energy-dispersive X-ray spectroscopy(EDX) were applied for the investigation of the microscopic structure and surface elements of the dressings as well as mechanical properties, water absorption, cell compatibility and antibacterial function so that we could comprehensively evaluate the functionality and practicability of dressings. The results show that the fibers of dressing is a crossing grid-like structure, interconnected, and gradually enhenced the mechanical strength of fibers with increasing of n-Ag content, as well as water absorption ability. When the n-Ag content is more than 1%(mass fraction), the dressings show a good antibacterial ability against the Gram-positive staphylococci and Gram-negative Escherichia coli. Cell experimental results show that when the content of n-Ag is between 0.01%—10%, the dressings are non-toxic or low toxicity; when the content of n-Ag is less than 1%, it contributes to the growth and proliferation of cells. Therefore, we conclude that the dressing has a good biocompatibility and antibacterial properties, and has broad application prospects in a functional medical dressings research and industrial area.

Key words: Electrospinning, Nano-silver, Poly(lactide-co-glycolide), Dressing

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