高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (3): 726.doi: 10.7503/cjcu20120550

• 高分子化学 • 上一篇    下一篇

壳聚糖微图形的制备及表征

林虹, 谢超鸣, 鲁雄, 屈树新, 冯波, 翁杰   

  1. 西南交通大学材料科学与工程学院, 材料先进技术教育部重点试验室, 成都 610031
  • 收稿日期:2012-06-07 出版日期:2013-03-10 发布日期:2013-02-18
  • 通讯作者: 鲁雄,男,博士,教授,主要从事生物材料及微加工方面的研究.E-mail:luxiong_2004@163.com E-mail:luxiong_2004@163.com
  • 基金资助:

    国家自然科学基金(批准号:31070851)、教育部新世纪优秀人才计划基金(批准号:NCET-10-0704)、四川省杰出青年学术技术带头人资助计划(批准号:2011JQ0010)和中央高校基本科研业务费(批准号:SWJTU11CX150)资助.

Preparation and Characterization of the Chitosan Micropatterns

LIN Hong, XIE Chao-Ming, LU Xiong, QU Shu-Xin, FENG Bo, WENG Jie   

  1. Key Laboratory of Advanced Technologies of Materials(Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
  • Received:2012-06-07 Online:2013-03-10 Published:2013-02-18
  • Contact: Lu xiong E-mail:luxiong_2004@163.com

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被引次数

6

摘要：

通过微转移模塑法, 在硅片表面制备了圆形点阵、 方形点阵、 沟槽和波浪等4种形貌的壳聚糖(CS)微图形. CS微图形清晰规整, 成型效果良好. 成骨细胞在圆形点阵和方形点阵上借助伪足多攀附于点阵凸起处. 点阵图形对细胞增殖和分化无影响. 在沟槽和波浪微图形中, 成骨细胞大多被限制在沟区, 且沿着沟槽方向伸展. 沟槽和波浪微图形不利于细胞分化, 但能促进细胞增殖.

关键词: 微图形, 壳聚糖, 微转移模塑法, 成骨细胞

Abstract:

Micro-transfer molding was used to prepare four different chitosan(CS) micropatterns on silicon substrates: cylinder, square, groove and sine wave. The as-prepared CS micropatterns were well-formed. Osteoblasts were cultured on various micropatterns to evaluate the effects of micro-geometry on cell behavior. On micro-cylinders and micro-squares, osteoblasts attached on CS micro-dot arrays and displayed irregular morphologies with numerous filopodia-like extensions. These micro-dot arrays had no impact on cell proliferation and differentiation. On micro-grooves and sine wave micropatterns, cells located within the channels and aligned to the direction of channels. Micro-grooves and sine wave micropatterns were unfavorable to cell differentiation, whereas promoted cell proliferation.

Key words: Micropattern, Chitosan, Micro-transfer molding method, Osteoblast

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