高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (09): 1926-1931.doi: 10.3969/j.issn.0251-0790.2012.09.009

• 分析化学 • 上一篇    下一篇

基于中空氧化镍纳米微球和离子液体复合膜固定血红蛋白的NaNO2生物传感器

谷广哲1, 董社英1, 黄廷林1, 汤宏胜2, 郑建斌2   

  1. 1. 西安建筑科技大学理学院, 西安 710055;
    2. 西北大学分析科学研究所, 陕西省电分析重点实验室, 西安 710069
  • 收稿日期:2012-01-16 出版日期:2012-09-10 发布日期:2012-08-14
  • 通讯作者: 董社英,女,博士,教授,主要从事电分析化学研究.E-mail:dongsyy@126.com E-mail:dongsyy@126.com
  • 基金资助:

    国家自然科学基金(批准号: 50830303); 长江学者与创新团队发展计划(批准号: IRT0853)和西安建筑科技大学重大科技成果创新基金(批准号: ZC1004)资助.

NaNO2 Biosensor Based on Hollow Nano-nickel Oxide Microsphere and Ionic Liquid Composite Film Immobilizing Hemoglobin

GU Guang-Zhe1, DONG She-Ying1, HUANG Ting-Lin1, TANG Hong-Sheng2, ZHENG Jian-Bin2   

  1. 1. College of Sciences, Xi'an University of Architecture and Technology, Xi'an 710055, China;
    2. Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an 710069, China
  • Received:2012-01-16 Online:2012-09-10 Published:2012-08-14

摘要:

采用微波水热法合成了一种中空氧化镍纳米微球(NiO), 然后将其与1-丁基-3-甲基-咪唑四氟硼酸盐(BMIMBF4)的复合膜用于血红蛋白(Hb)在碳糊电极上的固定, 制备了NaNO2生物传感器. 通过扫描电子显微镜、 傅里叶变换红外光谱及紫外-可见光谱等分析表明, Hb已固定于NiO和BMIMBF4的复合膜中并能保持其生物活性; 进一步通过电化学阻抗法研究了修饰电极中混合物各组分的作用. 结果表明, 在NiO和BMIMBF4的复合膜中, Hb能实现有效的直接电子转移, 且修饰后的电极对NaNO2有良好响应, 响应时间小于5 s, 检出限为4.57 μmol/L(S/N=3), 灵敏度为46.2 μA·L·mmol-1, 线性范围为10~170 μmol/L, 表观米氏常数KM为2.4 mmol/L, 该方法的重现性和电极的稳定性良好.

关键词: 中空氧化镍纳米微球, 血红蛋白, 直接电化学, 亚硝酸钠, 生物传感器

Abstract:

Hollow nano-nickel oxide microsphere (NiO) was fabricated by microwave hydrothermal process and characterized by SEM. The prepared NiO was used to immobilize hemoglobin(Hb) on the surface of the carbon paste electrode with 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4). The structure of Hb in the composite film was still maintained native-like demonstrated by FTIR spectra and UV-visible spectra. Moreover, the functions of different components in the modified electrode were studied by electrochemical impedance spectroscopy. A pair of stable and quasi-reversible redox peaks was observed in phosphate buffer solution which indicating that heme-proteins could achieve its direct electron transfer effectively. The modified electrode displayed an excellent and rapid electrocatalytic response to the oxidation of NaNO2. The proposed biosensor exhibited a good linear response to the concentration of NaNO2 in the range of 10-170 μmol/L, with a detection limit of 4.57 μmol/L(S/N=3). The apparent Michaelis-Menten constant was estimated to be 2.4 mmol/L. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Key words: Hollow nano-nickel oxide microsphere, Hemoglobin, Direct electron transfer, NaNO2, Biosensor

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