高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (04): 714.doi: 10.3969/j.issn.0251-0790.2012.04.013

• 分析化学 • 上一篇    下一篇

富精氨酸多肽修饰的金纳米粒子跨膜传输

单洪岩1, 宇博1, 孙琳琳2, 王振新2   

  1. 1. 吉林大学化学学院, 长春 130023;
    2. 中国科学院长春应用化学研究所电分析国家重点实验室, 长春 130022
  • 收稿日期:2011-04-22 出版日期:2012-04-10 发布日期:2012-04-10
  • 作者简介:单洪岩, 男, 工程师, 主要从事分析化学研究. E-mail: shanhy@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20675080)资助.

Transmembrane Delivery of Rich Arginine Peptide Modified Gold Nanoparticles

SHAN Hong-Yan1, YU Bo1, SUN Lin-Lin2, WANG Zhen-Xin2   

  1. 1. College of Chemistry, Jilin University, Changchun 130023, China;
    2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2011-04-22 Online:2012-04-10 Published:2012-04-10
  • Supported by:

    国家自然科学基金(批准号: 20675080)资助.

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被引次数

1

摘要： 考察了富精氨酸多肽功能化的金纳米粒子作为载体对细胞外物质的跨膜传输行为. 通过生物素(Biotin)与亲和素(Streptavidin)的亲和反应将具有特定跨膜功能的富精氨酸RRRRRRRR(R8)多肽分子连接到多肽CALNN修饰的金纳米粒子表面, 实现粒子的功能化. 以荧光素为模型化合物, 利用激光共聚焦显微镜观察了纳米粒子的输送过程. 实验结果表明, 富精氨酸多肽功能化的金纳米粒子可以作为一种低毒高效的跨膜输送载体.

关键词: 金纳米粒子, 多肽, HeLa细胞, 跨膜传输

Abstract: The transmembrane behavior of rich arginine(R) peptide modified gold nanoparticles was studied. The functionalization of the gold nanoparticles was achieved by the conjugation of transmembrane peptide RRRRRRRR(R8) with peptide CALNN stabilized gold nanoparticle through the standard streptavidin-biotin chemistry. The transmembrane delivery of the model compound, fluorescein, was studied by confocal scanning laser microscope. The result suggests that the R8 modified gold nanoparticles can be used as an excellent vector for transporting extracellular materials into cells with low cellular cytotoxicity.

Key words: Gold nanoparticle, Peptide, HeLa cell, Transmembrane delivery

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