高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (01): 14.doi: 10.3969/j.issn.0251-0790.2012.01.002

• 研究论文: 无机化学 • 上一篇    下一篇

N,N'-双(4-氯苄基)-1,2-丙二胺合铜(Ⅱ)配合物的合成、晶体结构、抑菌活性及与BSA的相互作用

杨树平1, 韩立军2, 潘燕3, 王大奇4, 赵翠1, 王波1   

  1. 1. 淮海工学院化学工程学院, 连云港 222005;
    2. 淮海工学院数理学院, 连云港 222005;
    3. 北京大学医学部基础医学院药理系, 天然仿生药物国家重点实验室, 北京 100191;
    4. 聊城大学化学工程学院, 聊城 252059
  • 收稿日期:2011-08-16 出版日期:2012-01-10 发布日期:2011-12-20
  • 通讯作者: 杨树平,女,副教授,主要从事药物化学研究.E-mail:spyang69320@yahoo.cn E-mail:spyang69320@yahoo.cn
  • 基金资助:

    国家自然科学基金(批准号: 30901815)和淮海工学院自然科学基金(批准号: Z2009019)资助.

Synthesis, Crystal Structure, Antibacterial Activity and Interaction with BSA of Copper(Ⅱ) Complex with Ligand N,N'-Bis(4-chlorobenzyl)propane-1,2-diamine

YANG Shu-Ping1, HAN Li-Jun2, PAN Yan3, WANG Da-Qi4, ZHAO Cui1, WANG Bo1   

  1. 1. School of Chemical Engineering,Huaihai Institute of Technology, Lianyungang 222005, China;
    2. School of Mathematics and Science, Huaihai Institute of Technology, Lianyungang 222005, China;
    3. Department of Pharmacology, School of Basic Medical Sciences, Peking University and State Key Laboratory of Natural and Biomimetic Drugs, Beijing 100191, China;
    4. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China
  • Received:2011-08-16 Online:2012-01-10 Published:2011-12-20

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摘要： 合成了N,N'-双(4-氯苄基)-1,2-丙二胺铜(Ⅱ)配合物·(NH4)·Cl2, 通过元素分析和IR光谱对其进行了表征, 并通过X射线单晶衍射确定了其晶体结构. 晶体结构分析表明, 该晶体属于三斜晶系, P1空间群, 晶胞参数a=1.35222(16) nm, b=1.37899(17) nm, c=1.39806(19) nm; α=60.954(1)°, β=87.502(2)°, γ=65.970(1)°, V=2.0424(4) nm3, Dc=1.357 g/cm3, Z=2, F(000)=862, R1=0.0925, wR2=0.2668, S=1.001. 配合物的金属中心与来自2个配体的4个氮原子和1个末端氯原子配位, 形成了轻微扭曲的四方锥几何构型, 扭曲指数τ=0.04(1). 抗菌实验结果显示, 配合物对大肠杆菌、 枯草杆菌和金色葡萄球菌均表现出良好的抑菌作用. 采用荧光光谱研究了不同温度下配合物与牛血清白蛋白(BSA)的相互作用, 结果表明, 配合物对BSA 的荧光猝灭属于静态猝灭. 计算了不同温度下配合物与BSA间的结合常数(Ka), 结合位点(n≈1)及相关热力学参数(ΔH＞0, ΔS＞0, ΔG＜0), 结果表明, 二者主要靠疏水作用力结合. 依据Föster的非辐射能量转移理论, 求得给体(BSA)与受体(配合物)间的距离r=2.56 nm, 说明配合物与BSA 之间可能发生了非辐射能量转移.

关键词: 铜(Ⅱ)配合物, N,N’-双(4-氯苄基)-1,2-丙二胺, 晶体结构, 抑菌活性, 牛血清白蛋白

Abstract: A new copper(Ⅱ) complex with ligand N,N'-bis(4-chlorobenzyl)propane-1,2-diamine, ·NH4·Cl2, has been synthesized and characterized by elemental analysis, IR spectra and single crystal X-ray diffraction methods. The crystal of copper(Ⅱ) complex belongs to triclinic system with space group P1, a=1.35222(16)nm, b=1.37899(17)nm, c=1.39806(19)nm; α=60.954(1)°, β=87.502(2)°, γ=65.970(1)°, V=2.0424(4) nm3, Dc=1.357 g/cm3, Z=2, F(000)=862, R1=0.0925, wR2=0.2668, S=1.001. The copper(Ⅱ) atom of the metal center in complex is five-coordinated by four N atoms from two ligand molecules and a terminal Cl atom, and the coordination polyhedron around the Cu atom can be described as a slightly distorted square pyramid-geometry with a distortion index τ value of 0.04(1). The complex exhibited good antibacterial properties and the interaction of complex and bovine serum albumin(BSA) was investigated by fluorescence spectroscopy at different temperatures. The results indicated that the complex caused the fluorescence quenching of BSA through a static quenching procedure. The binding constants(Ka), the number of binding sites(n≈1), and corresponding thermodynamic parameters enthalpy change(ΔH＞0), entropy change(ΔS＞0), Gibbs free energy change(ΔG＜0), between complex and BSA at different temperatures were calculated. The primary binding pattern between complex and BSA was interpreted as hydrophobic interaction. The binding average distance r between the donor(BSA) and acceptor(complex) was obtained to be 2.56 nm based on the Föster's theory, which indicates the energy transfer can occur from BSA to complex with high probability.

Key words: Copper(Ⅱ) complex, N,N’-bis(4-chlorobenzyl)propane-1,2-diamine, Crystal structure, Antibacterial activity, Bovine serum albumin(BSA)

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