A hydrolysable cationic polycarboxybetaine ester polymer brush(PCBMA-1C2) was fabricated by photografting method with surface-coupled tertiary amine as hetergenous hydrogen donor and benzophenone(BP) as photosensitizer. The hydrolysis of PCBMA-1C2 modified surface was evaluated by studying the hydrolysis behavior of CBMA-1C2 monomer under different ammonium hydroxide concentrations. X-ray photoelectron spectroscopy(XPS) and water contact angle were used to monitor the chemical changes and hydrophilicity, respectively. The protein adsorption and platelet adhesion experiments were used to evaluate the interaction between the material surface and the biomolecules. The results show that when the concentrations of ammonium hydroxide are 0.1, 0.2, 0.3 and 0.4 mol/L, the degrees of hydrolysis(DH) of terminal ester of CBMA-1C2 monomer are 6%, 43%, 56% and almost 100%, respectively. Compared with Glass-PCBMA-1C2 surface, with the increase of DH, the protein adsorption of BSA are reduced by 3%, 76%, 93% and 96%, and that of Fg are reduced by 11%, 45%, 90% and 96%, respectively. When DH>50%, the hydrolyzed Glass-PCBMA-1C2 surface exhibits good resistance of protein adsorption and platelet adhesion.