Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (5): 1044.doi: 10.7503/cjcu20131144
• Physical Chemistry • Previous Articles Next Articles
WU Yao, LIANG Wenjing, LI Cheng, SHANG Xu, CONG Lina*()
Received:
2013-11-25
Online:
2014-05-10
Published:
2014-04-18
Contact:
CONG Lina
E-mail:congln@dlpu.edu.cn
Supported by:
CLC Number:
TrendMD:
WU Yao, LIANG Wenjing, LI Cheng, SHANG Xu, CONG Lina. Effects of Temperature on the Antibacterial Activity and Structural Change of the C-terminal Polypeptide of the Sea Cucumber Lysozyme†[J]. Chem. J. Chinese Universities, 2014, 35(5): 1044.
Fig.1 Map of the recombination plasmid(A) and the expression of recombination SjLys-C on SDS-PAGE(B)^M: Protein markers; lane 1: control; lane 2: total cellular protein; lane 3: sample in precipitation after ultrasonication; lane 4: sample in supernatant after ultrasonication; lane 5: recombinant SjLys-C after Ni2+-NTA affinity chromatography; lane 6: recombinant SjLys-C after G-25 desalination column.
Tested bacteria | Diameter of inhibition zonea/mm | |||
---|---|---|---|---|
Rosetta(DE3)pLysSb | Rosetta-gamiB(DE3)pLysS | |||
SjLys-C | Heat-treated SjLys-C | SjLys-C | Heat-treated SjLys-C | |
M. lysodeikticus | 19 | 23 | 18 | 22 |
S. aureus | 10 | 10 | 19 | 19 |
P. aeruginosa | 8 | 8 | 14 | 15 |
V. parahaemolyticus | 20 | 21 | 20 | 21 |
Table 1 Antimicrobial activities of the recombinant SjLys-C expressed in different host strains
Tested bacteria | Diameter of inhibition zonea/mm | |||
---|---|---|---|---|
Rosetta(DE3)pLysSb | Rosetta-gamiB(DE3)pLysS | |||
SjLys-C | Heat-treated SjLys-C | SjLys-C | Heat-treated SjLys-C | |
M. lysodeikticus | 19 | 23 | 18 | 22 |
S. aureus | 10 | 10 | 19 | 19 |
P. aeruginosa | 8 | 8 | 14 | 15 |
V. parahaemolyticus | 20 | 21 | 20 | 21 |
Fig.6 Comparision of the structures from simulated annealing^The native sructures were selected from the MD structure of SjLys-C at 100 ℃ at different time points 5, 6, 7, 8, 9 and 10 ns.
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