Amperometric Mediated Biosensor Based on Tetrathiafulvalene as Electron Shuttle Between Glucose Oxidase and Glassy Carbon

Abstract

Abstract: Amperometric mediated biosensor for glucose has been developed by using aqueous Nafion to immobilize glucose oxidase on Nafion-TTFmodlfied glassy carbon. It is further coated by Nafion, a perfluorosulphonated ion-exchange polymer. Nafion coating is not detrimental to the enzyme but to forestall electrochemically active anionic interferences such as ascorbate and urate from reaching the electrode.The polymer coating also avoids tetrathiafulvalene's leaking and keeps the biosensor from fouling agents. For the first time it has been experimentally proved that both TTF⁺ and TTF²⁺ can oxidise FADH₂ of glucose oxidase. The biosensor responds rapidly to glucose in less than₂₀ seconds and it's calibration curve is linear from 3.0×10^-4 to 1.0×10^-2 mol/L.

Key words: Biosensor, Aqueous Nafion, Tetrathiafulvalene, Mediator, Glucose oxidase

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LIU Hai-Ying, LEI Cheng-Hong, DENG Jia-Qi . Amperometric Mediated Biosensor Based on Tetrathiafulvalene as Electron Shuttle Between Glucose Oxidase and Glassy Carbon[J]. Chem. J. Chinese Universities, 1994, 15(12): 1757.

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Amperometric Mediated Biosensor Based on Tetrathiafulvalene as Electron Shuttle Between Glucose Oxidase and Glassy Carbon

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