Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite

Abstract

Abstract: Experimental studies on the effects of the concentration of PO₄^3- and Ca ²⁺, ion strength and pHof buffer on the polarity and magnitude of F-potential of hydroxylapatite(HAP) were carried out in present study to characterize the surface physicochemical properties of hydroxylapatite. Adsorption of bovine serum albumin(BSA) on HAPwas carried at different conditions in terms of pH, ionic strength, and the concentration of PO₄^3- to establish the knowledge of adsorption mechanism. A Langmuir adsorption isotherm was obtained and the apparent activation energy and adsorption heat were interpreted. The low magnitude of the apparent activation energy and the adsorption heat indicated the fast adsorption of BSAon hydroxylapatite was a physical adsorption in nature.

Key words: Hydroxylapatite, Bovine serum albumin, Zeta potential, Adsorption, Kinetics

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YIN Gang, ZHAN Jin, LIU Zheng, YUAN Nai-Ju . Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite[J]. Chem. J. Chinese Universities, 2001, 22(5): 771.

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Characterization of the Adsorption of Bovine Serum Albumin on Hydroxylapatite

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