Plant Tissue-based Chemiluminescence Flow Biosensor for the Determination of Oxalate

Abstract

Abstract: Anovel chemiluminescence(CL) biosensor for the determination of oxalate is presented in this paper. It is based on the CLreaction between luminol, immobilized on an anion-exchange column, and hydrogen peroxide generated on-linevia oxalate by the catalytic effect of oxalate oxidase in spinach in the flow cell. The biosensor responds linearly to oxalate concentration in the range of 4.0×10^-7 to 1.0×10^-4 mol/L, and the detection limit is 2×10^-7 mol/L. Acomplete analysis could be performed in 1 min with a relative standard deviation of 3.7% for 1.0×10^-5 mol/Loxalate(n=7). The system is stable for over 200 times determinations and has been applied in urine samples.

Key words: Biosensor, Flow injection, Chemiluminescence, Oxalate

CLC Number:

O657.3

TrendMD:

PENG You-Yuan, QIN Wei, ZHANG Zhu-Jun . Plant Tissue-based Chemiluminescence Flow Biosensor for the Determination of Oxalate[J]. Chem. J. Chinese Universities, 2001, 22(2): 215.

[1]	WANG Ruina, SUN Ruifen, ZHONG Tianhua, CHI Yuwu. Fabrication of a Dispersible Large-sized Graphene Quantum Dot Assemblies from Graphene Oxide and Its Electrogenerated Chemiluminescence Behaviors [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220161.
[2]	WANG Junyang, LIU Zheng, ZHANG Qian, SUN Chunyan, LI Hongxia. Application of DNA Silver Nanoclusters in the Fluorescence Biosensors based on Functional Nucleic Acids [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220010.
[3]	SONG Youwei, AN Jiangwei, WANG Zheng, WANG Xuhui, QUAN Yanhong, REN Jun, ZHAO Jinxian. Effects of Ag，Zn，Pd-doping on Catalytic Performance of Copper Catalyst for Selective Hydrogenation of Dimethyl Oxalate [J]. Chem. J. Chinese Universities, 2022, 43(6): 20210842.
[4]	DU Shunfu, WANG Wenjing, EL⁃SAYED El⁃Sayed M., SU Kongzhao, YUAN Daqiang, HONG Maochun. A Chemiluminescent Zirconocene Coordination Tetrahedron [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210628.
[5]	WU Yangyi, CHEN Jianping, Ai Yijing, WANG Qingxiang, GAO Fei, GAO Feng. Synthesis of 2-（2-Hydroxy-3-methoxyphenyl）-C₆₀ and Its Application for Sensing of Cauliflower Mosaic Virus 35S Promotor [J]. Chem. J. Chinese Universities, 2021, 42(6): 1754.
[6]	XU Mengyi, HUANG Xuewen, LI Xiaojie, WEI Wei, LIU Xiaoya. Fabrication of Biosensor Based on “Beads-on-a-String” Shaped Composite Nano-assembly Modified Screen Printed Electrode [J]. Chem. J. Chinese Universities, 2021, 42(6): 1768.
[7]	WANG Bodong, PAN Meichen, ZHUO Ying. Construction of Electrochemiluminescence Sensing Interface Based on Silver Nanoclusters-Silica Nanoparticles and Biomolecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3519.
[8]	ZHANG Jiayi, DING Zhenyao, WANG Dandan, CHEN Liping, FENG Xinjian. Fabrication of Triphase Enzyme Electrode Based on Porous Gold Substrate for High-performance Electrochemical Biosensor [J]. Chem. J. Chinese Universities, 2021, 42(10): 3167.
[9]	LIAO Ni, ZHANG Jieyuan, HUANG Ziyang, ZHAO Yanxi, CHAI Yaqin, YUAN Ruo, ZHUO Ying. Construction of High Efficiency Uric Acid Sensor Based on the co-Crystal Enhanced Electrochemiluminescence from 9,10-Diphenylanthracene-perylene Microcrystals [J]. Chem. J. Chinese Universities, 2020, 41(9): 1989.
[10]	CAO Zhiyuan, SUN Hui, SU Bin. Electrochemiluminescence of Quantum Dots: Research Progress and Future Perspectives [J]. Chem. J. Chinese Universities, 2020, 41(9): 1945.
[11]	LIU Hong,ZOU Guojun,SUN Xinyuan,OUYANG Jianming. Differences of Growth and Cytotoxicity of Calcium Oxalate Crystals Formed on HK-2 Cells Under Different Oxalic Acid/Calcium Ratios ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1231.
[12]	YUAN Zhongwen, HE Lizhen, CHEN Tianfeng. Biomedical Applications of Single-atom Catalysts [J]. Chem. J. Chinese Universities, 2020, 41(12): 2690.
[13]	ZHANG Yimeng, ZHANG Huixin, LIU Yang. Recent Advances of Exosomes Bioanalysis and Their Clinic Applications [J]. Chem. J. Chinese Universities, 2020, 41(11): 2306.
[14]	ZHANG Jingjing, JIN Rong, FANG Danjun, JIANG Dechen. Voltage Modulated Electrochemiluminescence for Highly Sensitive Detection [J]. Chem. J. Chinese Universities, 2020, 41(11): 2421.
[15]	ZHENG Shan, LIU Yang, CHEN Piaopiao, XING Yichen, HUANG Chaobiao. Novel Glutathione Photoelectrochemical Sensor Based on PbS QDs/TiO₂ NPs ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1866.

Plant Tissue-based Chemiluminescence Flow Biosensor for the Determination of Oxalate

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments