Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (2): 210.

• Articles • Previous Articles     Next Articles

Chromatographic Properties of a Novel Shielded Bonded Stationary Phase for Liquid Chromatography

JING Yu-Qing, LONG Yuan-De, HUANG Tian-Bao   

  1. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China
  • Received:2000-11-09 Online:2002-02-24 Published:2002-02-24

Abstract: A novel bonded stationary phase, octadecanamido imine bonded stationary phase(ODAI), for reversed-phase HPLC was prepared by bonding stearyl chloride to YWG-80 silica gel through 3-(2-aminoethylamino)propyltrimethoxysilane. Hydrophobicity, selectivity and silanophilic activity of ODAIphase were evaluated by using aromatic compounds as analytes and methanol-water as binary mobile phase. The organic components including acidic, basic, neutral aromatic analytes could be separated satisfactorily with an excellent selectivity and chromatographic peak shape. The asymmetry factors of basic aniline, o-toluidine and N,N-dimethylaniline were found to be 114, 106 and 101, respectively, using methanol-water(volume ratio is 55:45) as mobile phase. Aniline is eluted before phenol due to the internal masking interaction to suppress ion exchange activity of residual silanols.

Key words: High-performance liquid chromatography, Stationary phase, Silanophilic activity, Alkylamide, Imine

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