Studies on Enzymological Properties of Antibody Elicited bymeso-Tetra(α, α, α, α-O-phenylacetyl benzene)porphyrin

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (4): 530.

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Studies on Enzymological Properties of Antibody Elicited bymeso-Tetra(α, α, α, α-O-phenylacetyl benzene)porphyrin

QI Chao^1,2, DING Lan¹, DING Tian-Bing³, LIU Zhi-Qiang¹, ZHANG Yu-Jing², LIU Li-Yan¹, ZHAO Da-Qing¹

1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Biochemistry Section of Basic Department, Quartermaster University of PLA, Changchun 130062, China;
3. Key Laboratory of Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130023, China

Received:2001-05-09 Online:2002-04-24 Published:2002-04-24

Abstract

Abstract: meso-Tetra(α,α,α,α-O-phenylacetyl benzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques.McAb1F2 obtained was demonstrated very pure by HPLCand MALDI/TOFMS.The retention time of McAb1F2 was 2.63 min.The subtype of McAb1F2 was IgG2a.The relative molecular weight was 156678.8.When the McAb1F2-porphyrin was formed, the maximal absorption of the porphyrin soret region had a redshift from 408 to 416 nm and hyperchromical effect, showing that the antigen-antibody combination was rigid and intense , and the abzyme constancy was high.But compared with HRP, the activity of the abzyme was only 4.6875 U/mg and 1.899% of that of HRP.Its K_m was 20.29 mmol/L, k_cat 396.82 min^-1, k_cat/K_m 1.9557×10⁴ L·mol^-1·min^-1.

Key words: meso-Tetra (α,α,α,α-O-phenylacetyl benzene)porphyrin, McAb, Complete antigen, Peroxidase

CLC Number:

Q554

TrendMD:

QI Chao, DING Lan, DING Tian-Bing, LIU Zhi-Qiang, ZHANG Yu-Jing, LIU Li-Yan, ZHAO Da-Qing. Studies on Enzymological Properties of Antibody Elicited bymeso-Tetra(α, α, α, α-O-phenylacetyl benzene)porphyrin[J]. Chem. J. Chinese Universities, 2002, 23(4): 530.

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Studies on Enzymological Properties of Antibody Elicited bymeso-Tetra(α, α, α, α-O-phenylacetyl benzene)porphyrin

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