Synthesis and Characteristic of Double Model Molecule Probe Targeting Myeloperoxidase

doi:10.7503/cjcu20170817

Abstract

Abstract:

5-Hydroxytryptamine(5-HT), a targeting molecule for myeloperoxidase from macrophages, was grafted on to (PIA-g-PEG-g-DDA) to prepare a type of amphiphilic polymers with targeting function(5-HT-g-PIA-g-PEG-g-DDA). Superaramagnetic iron-oxide nanoparticles(SPIONs) and fluorescent C dots(CDs) were decorated to the polymers for double-modal imaging. The size and morphology of the obtained nanoprobe were determined. Fluorescence spectrophotometer and vibrating sample magnetometer(VSM) were used to confirm the imaging capability of these bimodal nanoparticles. Confocal laser scanning microscope(CLSM) showed that the nano reagent could target macrophages in atherosclerotic plaque effectively and good biocompatibility was verified by 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumromide(MTT). The results suggested that the double-modal nano reagent has good targeting ability, high resolution, convenience and is able to become an effective probe for atherosclerosis.

Key words: Double model probe of nuclear magnetic resonance imaging and fluorescent powder imaging, Myeloperoxidase, 5-Hydroxytryptamine, Nanoparticles, Atherosclersis

CLC Number:

O631

TrendMD:

LIU Ye, YAO Shunyu, FANG Chao, ZHAO Waiou, WANG Jingyuan, LI Yapeng. Synthesis and Characteristic of Double Model Molecule Probe Targeting Myeloperoxidase[J]. Chem. J. Chinese Universities, 2018, 39(7): 1573.

Figures/Tables 11

Scheme 1 Synthesis of CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION

Fig.1 1H NMR spectra of PIA-g-PEG-g-DDA(A)and 5-HT-g-PIA-g-PEG-g-DDA(B)

Fig.2 TEM image of initial 5-HT-g-PIA-g-PEG-g-DDA@SPION(A) and CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION(B)^ Inset is the enlargement of (A).

Fig.3 DLS spectra of 5-HT-g-PIA-g-PEG-g-DDA@SPION(A) and CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION(B)

Fig.4 TGA curve of CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION at 10 ℃/min

Fig.5 MRI test for CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION

Fig.6 Red shift(A), the influence of concentration for intensity of fluorescence(B) and 3D fluorescence spectrum(C) of CDs@5-HT-g-PIA-g-PEG-g-DDA^ c(Fe)/(g·mL-1): (A) a. 0.01; b. 0.05; c. 0.10. (B) a. 0.2 ; b. 0.3; c. 0.4; d. 0.5.

Fig.7 Characteristic of CDs@5-HT-g-PIA-g-PEG-g-DDA fluorescence in macro under 365 nm UV lamp right(A) and under day light(B)

Fig.8 VSM analysis of SPION(A) and CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION(B)

Fig.9 Ccytotoxicity test of CDs@5-HT-PIA-g-PEG-g-DDA@SPION

Fig.10 Cell uptake test of CDs@5-HT-g-PIA-g-PEG-g-DDA@SPION^(A1—A4) were for cell of RAW264.7 under probe; (B1—B4) were free the probe in the cell of RAW264.7; (C1—C4) were for contrast of the endotheliocyte under probe; (D1—D4) were free the probe in the endotheliocyte. (A1—D1) DAPI; (A2—D2) FACIN; (A3—D3) polmer; (A4—D4) MERGE.

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