Cascade Biodegradation of Organic Pesticide Isoproturon

doi:10.7503/cjcu20170055

Abstract

Abstract:

Cascade biodegradation of organic pesticide isoproturon by enzymatic oxidation and activated sludge treatment is presented in this work. An enzymatic approach for the degradation of pesticide isoproturon catalyzed by chloroperoxidase with H₂O₂ as oxidant was proposed firstly. The complete degradation efficiency was achieved within 10 min at pH=3.0 and room temperature. The required H₂O₂ concentration was 0.2 mmol/L, and the dosage of chloroperoxidase(CPO) was only 7 nmol/L, indicating very few enzyme was required. The proposed degradation method was very efficient, mild, environment-friendly and no second pollution was involved, ensuring a potential application. The degradation products of isoproturon by CPO were determined by HPLC-MS, and the degraded route was proposed accordingly. Isoproturon was decomposed into small molecule 4-isopropyl aniline and 4-ethyl aniline benzylic cation by CPO-H₂O₂ catalytic oxidation. If the CPO-H₂O₂ was used as pre-stage treatment and was combined with activated sludge, the chemical oxygen demand(COD) can be eliminated to achieve effective decontamination of waste water containing isoproturon. Moreover, the ecotoxicity test using a green algae, Chlorella pyrenoidosa, showed that the growth inhibition ratio decreased with the increase of degradation of efficiency of isoproturon, indicating that the degraded products had lower toxicity than the parent isoproturon molecule.

Key words: Chloroperoxidase, Isoproturon, Cascade biodegradation, Products determination, Eco-toxicity evaluation

CLC Number:

O629

TrendMD:

WANG Fengjiao, LI Xuelian, HU Mancheng, LI Shuni, ZHAI Quanguo, JIANG Yucheng. Cascade Biodegradation of Organic Pesticide Isoproturon[J]. Chem. J. Chinese Universities, 2017, 38(8): 1362.

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