Preparation of Rare Earth Ion Induced Conformation-specificAnti-calmodulin Monoclonal Antibody

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (4): 526.

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Preparation of Rare Earth Ion Induced Conformation-specificAnti-calmodulin Monoclonal Antibody

LI Wei-Guo¹, QI Chao^1,2, Du Li², LIU Zi², ZHAO Da-Qing¹

1. Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Department of Biochemistry, Quartermaster University of PLA, Changchun 130062, China

Received:2001-06-28 Online:2002-04-24 Published:2002-04-24

Abstract

Abstract: Monoclonal antibody technique was employed to detect the conformational change of calmodulin induced by metal ions.Bovine calmodulin was firstly modified by 2,4-dinitrofluorobenzene to improve its immunogenicity, then, the derived protein was saturated with trivalent europium ions and injected to Balb/c mice as antigen.After four times of immunization, a corresponding antibody was detected and its titer in serum was determined as 1:12000.By fusing of the spleen cells with hybridoma cells, a europium induced conformation-specific anti-calmodulin monoclonal antibody cell strain named as 2C3 was produced successfully.The molecular recognition ability of antibody to apocalmodulin and holocalmodulin showed a significant difference, indicating that this antibody could be applied to the studies of different effects of metal ions on the conformational change of calmodulin and its interaction with target molecules.

Key words: Calmdoulin protein, Rare earth ion, Monoclonal antibody

CLC Number:

Q939.91

TrendMD:

LI Wei-Guo, QI Chao, Du Li, LIU Zi, ZHAO Da-Qing. Preparation of Rare Earth Ion Induced Conformation-specificAnti-calmodulin Monoclonal Antibody[J]. Chem. J. Chinese Universities, 2002, 23(4): 526.

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Preparation of Rare Earth Ion Induced Conformation-specificAnti-calmodulin Monoclonal Antibody

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