Investigation of Fibroin Composition of Bombyx Mori Silk Proteins by SDS-PAGE

Abstract

Abstract: The fibroin proteins directly extracted from silk glands of Bombyx mori were used for polyacrylamide gel electrophoresis in th presence of sodium dodecyl sulfate (SDS-PAGE).The subunit structure of fibroin molecule was discussed.It has been elucidated that the native fibroin molecule consists of subunits a,b and c with molecular weights of 280 kD, 230 kD and 25 kD, respectively, Since the amount of them is much more than that of the b subunit, the a and b subunits are the major component of fibroin.Of which the b subunit is composed of smaller e subunit of M_w about 130 kDand f subunit of M_W about 125 kD,linked by disulfide bonds.

Key words: Fibroin protein, Subunit structure, Polyacrylamide gel, Electrophoresis

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YU Tong-Yin, CAI Zai-Sheng, HUANG Wei-Da. Investigation of Fibroin Composition of Bombyx Mori Silk Proteins by SDS-PAGE[J]. Chem. J. Chinese Universities, 1996, 17(5): 829.

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Investigation of Fibroin Composition of Bombyx Mori Silk Proteins by SDS-PAGE

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