Capillary Zone Electrophoresis Separation of Basic Proteins Using Arginine and Lysine as Buffer Additives

Abstract

Abstract: The separation of basic proteins by capillary electrophoresis was plagued by the adsorption of the basic proteins onto the capillary inner surface,Atnethod is presented to solve this problem by using arginine and lysine as buffer additives,It was found that in the neutral or slightly alkaline solution, arginine and lysine can be adsorbed onto the inner surface of capillary to reduce the electroosmotic flow and the adsorption of the proteins efficiently, which results in improvement of resolution efficiency and reproducibility of the migration time and peak height of basic proteins,The experimental results show that arginine and lysine are the efficient buffer additives used in separation of basic proteins.

Key words: Capillary electrophoresis, Basic proteins, Adsorption, Arginine, Lysine

TrendMD:

REN Ji-Cun, DENG Yan-Zhuo, CHENG Jie-Ke. Capillary Zone Electrophoresis Separation of Basic Proteins Using Arginine and Lysine as Buffer Additives[J]. Chem. J. Chinese Universities, 1996, 17(7): 1059.

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Capillary Zone Electrophoresis Separation of Basic Proteins Using Arginine and Lysine as Buffer Additives

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