Abstract: Identification of gallbladder cancer rapidly at the time of surgery would allow the appropriate resection to be completed at the original surgery. The aim of this study was to assess the sensitivity and specificity of FTIR in the rapid detection of gallbladder cancer, via an attenuated total reflection(ATR) accessory with a ZnSe window. This allowed the edges of the surgically resected specimens to be examined rapidly. The results indicate that there are a series of differences between the spectra of normal and malignant tissues, and subtraction technique is helpful to identify cholecystitis and gallbladder cancer. Compared with pathological diagnosis results, one benign specimen was classified as a malignant and 6/6 malignant specimens and 39 cases of benign specimens were correctly classified. The sensitivity was 100%(6/6), specificity 97%(39/40), and accuracy was 97%(45/46). We can conclude that mid-infrared ATR FTIR spectroscopy is highly accurate and rapidly allows appropriately tailored resection at the time of initial surgery.

Key words: FTIR, Mid-IR fiber optics, Gallbladder, Detection