Composition and Antioxidative Actvity of Polysaccharide from Bergamot

Abstract

Abstract: After being extracted with hot water and precipitated with alcohol, crude polysaccharide was obtained from bergamot. Crude bergamot polysaccharide was gotten rid of proteins then isolated and purified by DEAE-cellulose. Two fractions were obtained and named BP1 and BP2. BP1 and BP2 were shown to be homogeneous on gel filtration chromatogram and polyacrylamide gel electrophoresis(PAGE). The average molecular weights(M_w) of BP1 and BP2 were determined to be 17 773 and 65 589 respectively, by gel permeation chromatography. The components of them identified by TLC and HPLC were rhamnase, mannose, glucose, galactose and xylose. The effects of the polysaccharide on removal of reactive oxygenspecies in some modified chemical systems were investigated by chemiluminescence and spectrophotometry. The inhibition of the damage of DNA chain induced by hydroxyl radical by the bergamot polysaccharide was observed by chemiluminescence. The results show that the bergamot polysaccharide could efficiently remove O^-·₂, HO^. and significantly protected DNA chains from being damaged by free radicals.

Key words: Polysaccharides, Bergamot, Component anaysis, Antioxidant activity, Free radical

CLC Number:

O636.1

TrendMD:

ZHU Xiao-Yan, WU Jian-Min, JIA Zhi-Shen. Composition and Antioxidative Actvity of Polysaccharide from Bergamot[J]. Chem. J. Chinese Universities, 2005, 26(7): 1264.

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Composition and Antioxidative Actvity of Polysaccharide from Bergamot

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