A Study of In-line Dialysis Combined with Capillary Electrophoresis——Realization and Preliminary Applications of Capillary Electrophoresis with Dialytic Sampling Function

Abstract

Abstract: A novel method of in-line coupling of dialysis with capillary electrophoresis, which eliminates the influence of macromolecules and particles in sample matrixes during the analysis of small molecules, was established in this paper. A polysulfone membrane, which was prepared in the inlet end of the separation capillary by phase-inversion process, cut off macromolecules efficiently and durably, so the capillary can be used for clean-up sampling. Using this capillary in electrophoresis, little column efficiency lost was achieved and the RSD of migration times and peak areas of chlorpheniramine were 0.8% and 4.6%, respectively. During the continuous using of this capillary for 12 h, the performance of the membrane changed little. This technology was applied in the analysis of free caffeine in coffee milk, and with little interference, the concentration was determined to be 0.68 mmol/L. When this method was applied in interaction analysis, the binding constant of promethazine hydrochloride with bovine serum albumin was obtained, that is 1.47×10⁴ L/mol.

Key words: Dialysis, Capillary electrophoresis, Clean-up sampling

CLC Number:

O657

TrendMD:

YU Yan-Lin, CHEN Hua, XIAO Shang-You, LI Hong-Yi, TAO Ran, XIA Zhi-Ning. A Study of In-line Dialysis Combined with Capillary Electrophoresis——Realization and Preliminary Applications of Capillary Electrophoresis with Dialytic Sampling Function[J]. Chem. J. Chinese Universities, 2005, 26(2): 218.

References

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